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Maus IgM Isotyp-Kontrolle

ELISA, WB, SDS IgM unconjugated
Rockland
Produktnummer ABIN1045087
Hersteller Produkt- Nr.: 010-0107
  • Target Alle IgM Produkte
    IgM
    Wirt
    • 56
    • 37
    • 34
    • 5
    • 4
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    Maus
    Applikation
    ELISA, Western Blotting (WB), SDS-PAGE (SDS)
    Hersteller Produkt- Nr.
    010-0107
    Hersteller
    Rockland
    Verwendungszweck
    Mouse IgM Whole Molecule
    Kreuzreaktivität (Details)
    Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Mouse Serum and anti-Mouse IgM (μ chain specific).   No reaction was observed against anti-Mouse IgG F(c).  Some light chain cross-reactivity will occur with anti-Mouse IgG.
    Produktmerkmale
    Mouse Immunoglobulin M
    Aufreinigung
    Mouse IgM was prepared from normal serum by a multi-step process which includes delipidation, selective precipitation and tandem molecular sieve chromatography followed by extensive dialysis against the buffer stated above. 
    Sterilität
    Sterile filtered
    Isotyp
    IgM
  • Applikationshinweise

    Application Note: Mouse IgM whole molecule has been tested by SDS-Page and can be utilized as a control or standard reagent in SDS, Western Blotting, and ELISA experiments.

    Western Blot Dilution: user optimized

    ELISA Dilution: user optimized

    Other: User Optimized

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Liquid
    Konzentration
    1.16 mg/mL
    Buffer

    Buffer: 0.1 M Tris Chloride, 0.5 M Sodium Chloride, pH 8.0

    Stabilizer: None

    Preservative: 0.1 % (w/v) Sodium Azide
    Konservierungsmittel
    Sodium azide
    Vorsichtsmaßnahmen
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Lagerung
    4 °C,-20 °C
    Informationen zur Lagerung
    Store vial at 4° C prior to opening.   This product is stable 4° C as an undiluted liquid.  Dilute only prior to immediate use.  For extended storage mix with an equal volume of glycerol, aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing. 
    Haltbarkeit
    12 months
  • Target
    IgM
    Abstract
    IgM Produkte
    Substanzklasse
    Antibody
    Hintergrund
    Background: Mouse IgM, or mouse Immunoglobulin M, purified protein is a basic antibody that is produced by B cells. Mouse IgM is by far the physically largest antibody in the human circulatory system. Mouse IgM is the first antibody to appear in response to initial exposure to antigen. IgM is predominantly produced in the spleen. Mouse IgM is formed from covalently linking 5 immunoglobulins together, the approximate molecular weight of IgM is 900 kDa and possesses 10 binding sites.
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