(1 reference)-
- Target Alle IgG1 Produkte
- IgG1
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Wirt
- Maus
- Konjugat
- APC
- Applikation
- Flow Cytometry (FACS), Isotype Control (IsoC), Immunofluorescence (IF)
- Produktmerkmale
- This antibody can be used as a mouse IgG1 isotype control in flow cytometry and other applications.
- Isotyp
- IgG1
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Mouse IgG1 isotype control (Biotin)
FACS, ELISA, WB, IP, ICC, IHC (fro), IHC (p) Monoclonal MOPC-21 IgG1 kappa Biotin
Mouse IgG1 isotype control (FITC)FACS, ELISA Monoclonal 15H6 IgG1 FITC
Mouse IgG1 isotype control (PE)FACS, ELISA Monoclonal 15H6 IgG1 PE
Mouse IgG1 isotype controlFACS, ELISA, WB, IP, ICC, IHC (fro), IHC (p), Func Monoclonal MOPC-21 IgG1 kappa unconjugated
Mouse IgG1 isotype controlFACS, ELISA, WB, IP, ICC, IHC (fro), IHC (p) Monoclonal MOPC-21 IgG1 kappa unconjugated
Mouse IgG1 isotype control (SPRD)FACS, ELISA Monoclonal 15H6 IgG1 SPRD
Mouse IgG1 isotype control (APC)FACS, ELISA Monoclonal 15H6 IgG1 APC
Mouse IgG1 isotype control (Biotin)FACS, ELISA Monoclonal 15H6 IgG1 Biotin
Mouse IgG1 isotype control (PE-Cy7)FACS, ELISA Monoclonal 15H6 IgG1 PE-Cy7
Mouse IgG1 isotype control (APC-Cy7)FACS, ELISA Monoclonal 15H6 IgG1 APC-Cy7
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- Applikationshinweise
- Optimal working dilution should be determined by the investigator.
- Testdurchführung
-
- Take 100 μL peripheral blood anticoagulated by EDTA and add to the bottom of 5 mLtube,
- Add 20 μL labeled antibody to the bottom of flow tube mixing with the whole blood, incubate for 20 minutes at room temperature away from light,
- Add 2 ml1×RBC lysis buffer, incubate for 10 minutes away from light after mixing, dissolve red blood cells (recommended: RBC lysing Solution 10×,Cat.: FXP001),
- Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant,
- Add 2 mLPBS wash buffer to resuspend the cells, then1000 rpm centrifugation for 5 minutes, discard the supernatant,
- Add 0.5 mLPBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 °C then measured).
- [PBS wash buffer: PBS +1 % FBS +0.1 % NaN3, Cat.: FXP005]
- [Cell fixation: 2 % formaldehyde solution]
- Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Format
- Liquid
- Buffer
- Phosphate-buffered solution, pH 7.4, containing and 0.2 % (w/v) BSA
- Konservierungsmittel
- Sodium azide
- Vorsichtsmaßnahmen
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Lagerung
- 4 °C
-
-
: "The Expression Pattern of Surface Markers in Canine Adipose-Derived Mesenchymal Stem Cells." in: International journal of molecular sciences, Vol. 22, Issue 14, (2021) (PubMed).
-
: "The Expression Pattern of Surface Markers in Canine Adipose-Derived Mesenchymal Stem Cells." in: International journal of molecular sciences, Vol. 22, Issue 14, (2021) (PubMed).
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- Target
- IgG1
- Abstract
- IgG1 Produkte
- Synonyme
- IgG1 Isotype Control, Igh-4 Isotype Control, VH7183 Isotype Control, immunoglobulin heavy constant gamma 1 (G1m marker) Isotype Control, Ighg1 Isotype Control
- Substanzklasse
- Antibody
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