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Maus IgM Isotyp-Kontrolle

IsoC, FACS IgM unconjugated
Produktnummer ABIN2145425
  • Target Alle IgM Produkte
    IgM
    Wirt
    • 57
    • 37
    • 37
    • 5
    • 4
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    Maus
    Applikation
    Isotype Control (IsoC), Flow Cytometry (FACS)
    Produktmerkmale
    This antibody can be used as a mouse IgM isotype control in flow cytometry and other applications.
    Aufreinigung
    Purified
    Isotyp
    IgM
  • Applikationshinweise
    Optimal working dilution should be determined by the investigator.
    Testdurchführung
    • Take 100 μL peripheral blood anticoagulated by EDTA and add to the bottom of 5 mLtube,
    • Add appropriate amount of antibody to the bottom of flow tube mixing with the whole blood, incubate for 30 minutes at room temperature,
    • Add 2 ml1×RBC lysis buffer, incubate for 10 minutes after mixing, dissolve red blood cells (recommended: RBC lysing Solution 10×,Cat.: FXP001),
    • Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant,
    • Add 2 mLPBS wash buffer to resuspend the cells, then 1000 rpm centrifugation for 5 minutes, discard the supernatant,
    • Add appropriate amount of fluorescent-labeled anti-mouse IgGs and incubate for 20 minutes away from light at room temperature.
    • Repeat step 5.
    • Add 0.5 mLPBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 °C then measured).
    • [PBS wash buffer: PBS +1 % FBS +0.1 % NaN3, Cat.: FXP005]
    • [Cell fixation: 2 % formaldehyde solution]
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Liquid
    Buffer
    Phosphate-buffered solution, pH 7.4,
    Konservierungsmittel
    Sodium azide
    Vorsichtsmaßnahmen
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Lagerung
    4 °C
  • Target
    IgM
    Abstract
    IgM Produkte
    Synonyme
    IgM constant region Isotype Control, IGM Isotype Control
    Substanzklasse
    Antibody
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