ELISA, Flow Cytometry (FACS), Isotype Control (IsoC), Immunohistochemistry (IHC)
Produktmerkmale
To insure lot-to-lot consistency, each batch of product is tested to conform with characteristics of a standard reference reagent using immunofluorescence staining and analysis on a Becton Dickinson FACScan TM flow cytometer Purified antibody conjugate: <= 1 µg/10^6 cells, Fluorescein conjugate: 10 µL/10^6 cells, Biotin conjugate: <= 1 µg/10^6 cells, R-phycoerythrin conjugate: 10 µL/10^6 cells, Allophycocyanin conjugate: 10 µL/10^6 cells, Spectral Red conjugate: 10 µL/10^6 cells, R-phycoerythrin-Cyanine 5.
Each laboratory should determine an optimum working titer for use in its particular application. Other applications have not been tested but use in such assays should not necessarily be excluded.
Probenmenge
1 mL
Beschränkungen
Nur für Forschungszwecke einsetzbar
Buffer
Product is supplied as 1.0 mg of purified immunoglobulin in 1.0 mL of 100 mM borate buffered saline, pH 8.2.
Konservierungsmittel
Azide free
Handhabung
Each reagent is stable for the period shown on the bottle label if stored as directed.
Lagerung
4 °C
Alanne-Kinnunen, Lappalainen, Öörni, Kovanen: "Activated human mast cells induce LOX-1-specific scavenger receptor expression in human monocyte-derived macrophages." in: PloS one, Vol. 9, Issue 9, pp. e108352, (2015) (PubMed).