FLT1 ELISA Kit

Produktnummer ABIN1112705

Produktdetails FLT1 ELISA Kit

Target: FLT1 (Fms-Related tyrosine Kinase 1 (VEGFR1) (FLT1))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 156-10000 pg/mL

Untere Nachweisgrenze: 156 pg/mL

Applikation: ELISA

Verwendungszweck: For quantitative detection of VEGFR1 in human serum, plasma, body fluids, tissue lysates or cell culture supernatants.

Proben: Cell Culture Supernatant, Plasma, Serum, Tissue Lysate

Analytische Methode: Quantitative

Sensitivität: < 4 pg/mL

Bestandteile: 1. One 96-well plate pre-coated with anti-human VEGFR1 antibody 2. Lyophilized human VEGFR1 standards: 2 tubes (10ng / tube) 3. Sample / Standard diluent buffer: 30ml 4. Biotin conjugated anti-human VEGFR1 antibody (Concentrated): 130 µl.

Benötigtes Material: 1. 37 °C incubator 2. Microplate reader (wavelength: 450nm) 3. Precise pipette and disposable pipette tips 4. Automated plate washer 5. ELISA shaker 6. 1.5ml of Eppendorf tubes 7. Plate cover 8. Absorbent filter papers 9. Plastic or glass container with volume of above 1L

Anwendungsinformationen

Kommentare:

This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-VEGFR1 polyclonal antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-VEGFR1 polyclonal antibody was used as detection antibodies. The standards test samples and biotin conjugated detection antibody were added - the wells subsequently and wash with wash buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with wash buffer. TMB substrates were used - visualize HRP enzymatic reaction. TMB was catalyzed by HRP - produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional - the VEGFR1 amount of sample captured in plate. Read the O.D. absorbance at 450 nm in a microplate reader and then the concentration of VEGFR1 can be calculated.

Plattentyp: Pre-coated

Aufbereitung der Reagenzien:

1. Before the experiment, centrifuge each kit component for several minutes to bring down all reagents to the bottom of tubes. 2. It is recommend to measure each standard and sample in duplicate. 3. Do NOT let the plate completely dry at any time! Since the dry condition can inactivate the biological material on the plate. 4. Do not reuse pipette tips and tubes to avoid cross contamination. 5. Do not use the expired components and the components from different batches. 6. To avoid the marginal effect of plate incubation for temperature differences (the marginal wells always get stronger reaction), it is recommend to equilibrate the ABC working solution and TMB substrate for at least 30 min at room temperature (37°C ) before adding to wells.The TMB substrate (Kit Component 8) is colorless and transparent before use, if not, please contact us for replacement.

Aufbereitung der Proben:

Preparation of sample and reagents 1. Sample Isolate the test samples soon after collecting, then, analyze immediately (within 2 hours). Or aliquot and store at -20 °C for long term. Avoid multiple freeze-thaw cycles.
Tissue lysate or body fluids and cell culture supernatants: Centrifuge to remove precipitate, analyze immediately or aliquot and store at -20 °C .
Serum: Coagulate the serum at room temperature (about 4 hours). Centrifuge at approximately 1000 × g for 15 min. Analyze the serum immediately or aliquot and store at -20 °C .
Plasma: Collect plasma with EDTA as the anticoagulant. Centrifuge for 15 min at 1000 x g within 30 min of collection. Analyze immediately or aliquot and store frozen at -20 °C. Citrate and heparin can not be used as anticoagulant here. Note: 1. Coagulate blood samples completely, then, centrifuge, and avoid hemolysis and particle. 2. NaN3 can not be used as test sample preservative, since it is the inhibitor for HRP.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konservierungsmittel: Sodium azide, Thimerosal (Merthiolate)

Details zu FLT1

Target: FLT1 (Fms-Related tyrosine Kinase 1 (VEGFR1) (FLT1))

Andere Bezeichnung: VEGF-R1 (FLT1 Produkte)

Synonyme: FLT ELISA Kit, FLT-1 ELISA Kit, VEGFR-1 ELISA Kit, VEGFR1 ELISA Kit, FLT1 ELISA Kit, flt1b ELISA Kit, sFlt1 ELISA Kit, AI323757 ELISA Kit, Flt-1 ELISA Kit, fms related tyrosine kinase 1 ELISA Kit, FMS-related tyrosine kinase 1 ELISA Kit, vascular endothelial growth factor receptor 1 ELISA Kit, fms-related tyrosine kinase 1 (vascular endothelial growth factor/vascular permeability factor receptor) ELISA Kit, FMS-like tyrosine kinase 1 ELISA Kit, vascular endothelial growth factor receptor-1 ELISA Kit, FLT1 ELISA Kit, Flt1 ELISA Kit, CpipJ_CPIJ012087 ELISA Kit, flt1 ELISA Kit, FLT-1 ELISA Kit

Hintergrund: Vascular endothelial growth factor receptor 1 (VEGFR1), also known as FLT1, is a protein that in humans is encoded by the FLT1 gene, which maps to 13q12. Oncogene FLT belongs to the src gene family and is related to oncogene ROS. Like other members of this family, it shows tyrosine protein kinase activity that is important for the control of cell proliferation and differentiation. The deduced 1,338-amino acid protein has a calculated molecular mass of 150.6 kD. The sequence structure of the FLT gene resembles that of the FMS gene, hence, Yoshida et al. (1987) proposed the name FLT as an acronym for FMS-like tyrosine kinase.

Pathways: RTK Signalweg, Signaling Events mediated by VEGFR1 and VEGFR2, VEGFR1 Specific Signals