P-Cadherin ELISA Kit

Produktnummer ABIN1112776

Produktdetails P-Cadherin ELISA Kit

Target: P-Cadherin (CDH3)

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 62.5-4000 pg/mL

Untere Nachweisgrenze: 62.5 pg/mL

Applikation: ELISA

Analytische Methode: Quantitative

Sensitivität: < 2 pg/mL

Bestandteile: 1. One 96-well plate pre-coated with anti-Human P-Cadherin antibody 2. Lyophilized human P-Cadherin standards: 2 tubes (10ng / tube) 3. Sample / Standard diluent buffer: 30ml 4. Biotin conjugated anti-human P-Cadherin antibody (Concentrated): 130 µl.

Benötigtes Material: 1. 37 °C incubator 2. Microplate reader (wavelength: 450nm) 3. Precise pipette and disposable pipette tips 4. Automated plate washer 5. ELISA shaker 6. 1.5ml of Eppendorf tubes 7. Plate cover 8. Absorbent filter papers 9. Plastic or glass container with volume of above 1L

Anwendungsinformationen

Kommentare:

This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-P- Cadherin polyclonal antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-P-Cadherin polyclonal antibody was used as detection antibodies. The standards test samples and biotin conjugated detection antibody were added - the wells subsequently and wash with wash buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with wash buffer. TMB substrates were used - visualize HRP enzymatic reaction. TMB was catalyzed by HRP - produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional - the P-Cadherin amount of sample captured in plate. Read the O.D. absorbance at 450 nm in a microplate reader and then the concentration of P-Cadherin can be calculated.

Plattentyp: Pre-coated

Aufbereitung der Reagenzien:

1. Before the experiment, centrifuge each kit component for several minutes to bring down all reagents to the bottom of tubes. 2. It is recommend to measure each standard and sample in duplicate. 3. Do NOT let the plate completely dry at any time! Since the dry condition can inactivate the biological material on the plate. 4. Do not reuse pipette tips and tubes to avoid cross contamination. 5. Do not use the expired components and the components from different batches. 6. To avoid the marginal effect of plate incubation for temperature differences (the marginal wells always get stronger reaction), it is recommend to equilibrate the ABC working solution and TMB substrate for at least 30 min at room temperature (37°C ) before adding to wells.The TMB substrate (Kit Component 8) is colorless and transparent before use, if not, please contact us for replacement.

Aufbereitung der Proben:

Preparation of sample and reagents 1. Sample Isolate the test samples soon after collecting, then, analyze immediately (within 2 hours). Or aliquot and store at -20 °C for long term. Avoid multiple freeze-thaw cycles.
Tissue lysate or body fluids, cell culture supernate: Centrifuge to remove precipitate, analyze immediately or aliquot and store at -20 °C .
Serum: Coagulate the serum at room temperature (about 4 hours). Centrifuge at approximately 1000 × g for 10 min. Analyze the serum immediately or aliquot and store at -20 °C .
Plasma: Collect plasma with heparin, citrate or EDTA as the anticoagulant. Centrifuge for 10 min at 1000 x g within 30 min of collection. Analyze immediately or aliquot and store frozen at -20 °C. Note: 1. Coagulate blood samples completely, then, centrifuge, and avoid hemolysis and particle. 2. NaN3 can not be used as test sample preservative, since it is the inhibitor for HRP.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konservierungsmittel: Sodium azide, Thimerosal (Merthiolate)

Details zu P-Cadherin

Target: P-Cadherin (CDH3)

Andere Bezeichnung: P-Cadherin (CDH3 Produkte)

Synonyme: CDH3 ELISA Kit, Cadherin-3 ELISA Kit, AI385538 ELISA Kit, Cadp ELISA Kit, Cdhp ELISA Kit, P-cadherin ELISA Kit, Pcad ELISA Kit, CDHP ELISA Kit, HJMD ELISA Kit, PCAD ELISA Kit, E-Cad ELISA Kit, E-cadherin ELISA Kit, EP-cadherin ELISA Kit, XTCAD-1 ELISA Kit, c-cadherin ELISA Kit, cadherin-1 ELISA Kit, cdh1 ELISA Kit, cdh1-b ELISA Kit, cdh3 ELISA Kit, cdhc-A ELISA Kit, cdhp ELISA Kit, hjmd ELISA Kit, l-cam ELISA Kit, lcam ELISA Kit, pcad ELISA Kit, uvo ELISA Kit, uvomorulin ELISA Kit, xb-cad ELISA Kit, xcdh1 ELISA Kit, CDH15 ELISA Kit, K-CAM ELISA Kit, cadherin 3 ELISA Kit, cadherin 3 L homeolog ELISA Kit, Cadherin-3 ELISA Kit, CDH3 ELISA Kit, Cdh3 ELISA Kit, cdh3.L ELISA Kit, cdh-3 ELISA Kit

Hintergrund: Cadherin-3, also known as P-Cadherin, is a protein that in humans is encoded by the CDH3 gene. This gene is a classical cadherin from the cadherin superfamily. It is located in a six-cadherin cluster in a region on the long arm of chromosome 16 that is involved in loss of heterozygosity events in breast and prostate cancer. This protein is a calcium-dependent cell-cell adhesion glycoprotein composed of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. In addition, aberrant expression of this protein is observed in cervical adenocarcinomas. Mutations in this gene have been associated with congential hypotrichosis with juvenile macular dystrophy.