CMV IgG ELISA Kit

Produktnummer ABIN1326812

Produktdetails CMV IgG ELISA Kit

Target: CMV IgG (Anti-Cytomegalovirus IgG Antibody (CMV IgG))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Competition ELISA

Applikation: ELISA

Verwendungszweck: CMV IgG ELISA Kit is intended for the detection of IgG antibody to Cytomegalovirus (CMV) in human serum or plasma.

Proben: Plasma, Serum

Analytische Methode: Qualitative

Anwendungsinformationen

Applikationshinweise: Diluted patient serum is added to wells coated with purified antigen. IgG specific antibody, if present, binds to the antigen. All unbound materials are washed away and the enzyme conjugate is added to bind to the antibody-antigen complex, if present. Excess enzyme conjugate is washed off and substrate is added. The plate is incubated to allow the hydrolysis of the substrate by the enzyme. The intensity of the color generated is proportional to the amount of IgG specific antibody in the sample.

Plattentyp: Pre-coated

Ergebnisberechnung:

• Check Calibrator Factor (CF) value on the calibrator bottle. This value might vary from lot to lot. Make sure you check the value on every kit.
• Calculate the cut-off value: Calibrator OD x Calibrator Factor (CF).
• Calculate the Ab (Antibody) Index of each determination by dividing the O.D. value of each sample by cut-off value.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C

Referenzen für CMV IgG ELISA Kit (ABIN1326812)

Wang, Liu, Xu, Jackson, Roskin, Pham, Laserson, Marshall, Seo, Lee, Furman, Koller, Dekker, Davis, Fire, Boyd: "Effects of aging, cytomegalovirus infection, and EBV infection on human B cell repertoires." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 192, Issue 2, pp. 603-11, (2014) (PubMed).

Details zu CMV IgG

Target: CMV IgG (Anti-Cytomegalovirus IgG Antibody (CMV IgG))

Andere Bezeichnung: CMV IgG (CMV IgG Produkte)

Substanzklasse: Antibody

Hintergrund: Cytomegalovirus (CMV) is a member of the herpes group of viruses. Most adults and children who catch CMV have no symptoms and are not harmed by the virus. CMV infection is of clinical significance primarily in pregnant women, newborn infants with possible congenital infection, immunosuppressed transplant patients and iniduals with AIDS. CMV is so prevalent as over 60% of people catch the infection at some time in their lives.Significant increases in CMV IgG antibody by ELISA suggest recent infection or reactivation of a latent CMV infection.ELISA can detect CMV IgM antibody in both primary CMV infections (93-100%) and in reactivated infection (40%). An IgM response may be reduced or absent in immunocompromised patients with active infection. In transplant patients the CMV infection can be associated with higher morbidity and mortality.