(1 reference)-
- Applikation
- Cell Viability Assay (CVA)
- Marke
- CellCountEZ™
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Tumor Necrosis Factor alpha (TNF alpha) ELISA Kit
TNF alpha Reaktivität: Ratte Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Interleukin 1, beta (IL1B) ELISA KitIL1B Reaktivität: Ratte Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Tumor Necrosis Factor alpha (TNF alpha) ELISA KitTNF alpha Reaktivität: Maus Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
High Mobility Group Box 1 (HMGB1) ELISA KitHMGB1 Reaktivität: Human Colorimetric Sandwich ELISA 62.5 pg/mL - 4000 pg/mL Plasma, Serum
Lipopolysaccharides (LPS) ELISA KitReaktivität: Diverse Spezies Colorimetric Competition ELISA 12.35 ng/mL - 1000 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Vascular Cell Adhesion Molecule 1 (VCAM1) ELISA KitVCAM1 Reaktivität: Human Colorimetric Sandwich ELISA 1.563-100 ng/mL Plasma, Serum, Tissue Homogenate
Interleukin 6 (IL6) ELISA KitIL6 Reaktivität: Human Colorimetric Sandwich ELISA 7.8-500 pg/mL Cell Culture Supernatant, Plasma, Serum, Tissue Homogenate, Urine
Tumor Necrosis Factor alpha (TNF alpha) ELISA KitTNF alpha Reaktivität: Human AA 77-233 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (citrate), Plasma (heparin), Serum
Matrix Metalloproteinase 2 (MMP2) ELISA KitMMP2 Reaktivität: Human Colorimetric Sandwich ELISA 0.78 ng/mL - 50 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Interleukin 1, beta (IL1B) ELISA KitIL1B Reaktivität: Human Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
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- Applikationshinweise
- CellCountEZ™ Cell Survival Assay Kit contains enough reagents for 1000 assays using a 96-well plate. This assay gives a linear response for cells (0, 1.000, 5.000, 10.000, 20.000, 40.000) plated in 100 µl growth medium with up to 15% fetal bovine serum in a 96-well plate and measured 20 hours after plating. Drug toxicity could be measured for up to 4 days after treatment with drugs using this assay for 5.000 cells plated in 100 µl growth medium with up to 15% fetal bovine serum in a 96-well plate.
- Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Lagerung
- 4 °C
- Informationen zur Lagerung
- Store kit at 2-8° C prior to opening. See kit insert for complete instructions.
- Haltbarkeit
- See kit insert for complete instructions.
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: "Insights from HuR biology point to potential improvement for second-line ovarian cancer therapy." in: Oncotarget, Vol. 7, Issue 16, pp. 21812-24, (2017) (PubMed).
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: "Insights from HuR biology point to potential improvement for second-line ovarian cancer therapy." in: Oncotarget, Vol. 7, Issue 16, pp. 21812-24, (2017) (PubMed).
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- Hintergrund
- CellCountEZ™ Cell Survival Assay Kit is a tissue culture media-based assay that can measure metabolically active live cells and quantify cell death caused by radiation, chemotherapeutics or toxins. CellCountEZ™ is based on the ability of mammalian cells to rapidly and efficiently convert hydroxyethyl disulfide (HEDS) into mercaptoethanol (ME) through a bioreduction mechanism. Bioconversion of HEDS to ME relies on the activity of the oxidative pentose phosphate cycle (OPPC). CellCountEZ™ measures ME in the extracellular medium without the need for cellular lysis and extraction methods, this preserves the ability to perform other cellular tests in the same culture system. CellCountEZ™ is readily soluble, membrane permeable and converted by live cells intracellularly before transport into the extracellular culture media. CellCountEZ™ offers many advantages that make it superior to common existing methods for quantifying cell growth and survival. Synonyms: Toxicity, Proliferation, and Survival Assay, cell proliferation and survival, drug screening, cell viability, cell survival, cellular viability, cellular survival, HEDS assay.
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