Oxphos™ Cell Survival Assay Kit
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- Applikation
- Cell Viability Assay (CVA)
- Hersteller Produkt- Nr.
- kld-002
- Hersteller
- Rockland
- Marke
- Oxphos™
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Tumor Necrosis Factor alpha (TNF alpha) ELISA Kit
TNF alpha Reaktivität: Ratte Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Interleukin 1, beta (IL1B) ELISA KitIL1B Reaktivität: Ratte Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Tumor Necrosis Factor alpha (TNF alpha) ELISA KitTNF alpha Reaktivität: Maus Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
High Mobility Group Box 1 (HMGB1) ELISA KitHMGB1 Reaktivität: Human Colorimetric Sandwich ELISA 62.5 pg/mL - 4000 pg/mL Plasma, Serum
Amyloid beta 1-40 (Abeta 1-40) ELISA KitAbeta 1-40 Reaktivität: Human Colorimetric Sandwich ELISA 7.813 pg/mL - 500 pg/mL Plasma, Serum, Tissue Homogenate
Amyloid beta 1-42 (Abeta 1-42) ELISA KitAbeta 1-42 Reaktivität: Human Colorimetric Sandwich ELISA 4.688 pg/mL - 300 pg/mL Plasma, Serum, Tissue Homogenate
Lipopolysaccharides (LPS) ELISA KitReaktivität: Diverse Spezies Colorimetric Competition ELISA 12.35 ng/mL - 1000 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
High Mobility Group Box 1 (HMGB1) ELISA KitHMGB1 Reaktivität: Maus Colorimetric Sandwich ELISA 46.88 pg/mL - 3000 pg/mL Plasma, Serum
Vascular Cell Adhesion Molecule 1 (VCAM1) ELISA KitVCAM1 Reaktivität: Human Colorimetric Sandwich ELISA 1.563-100 ng/mL Plasma, Serum, Tissue Homogenate
Interleukin 6 (IL6) ELISA KitIL6 Reaktivität: Human Colorimetric Sandwich ELISA 7.8-500 pg/mL Cell Culture Supernatant, Plasma, Serum, Tissue Homogenate, Urine
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- Applikationshinweise
- Oxphos™ gives a linear response for mammalian cells (0, 100.000, 200.000, 400.000, 600.000, and 800.000) plated in 1mL growth medium in a six well plate with up to 15% fetal bovine serum and measured 20 hours after plating. It also gives a linear response for human blood (0, 10, 20, 40, 60, 80, 100 µL) suspended in a total volume of 200 µL saline in a microfuge tube and incubated with 10 µL of reagent 6 for 2hrs. Oxphos™ contains enough reagents for 100 assays using a a 6-well plate.
- Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Lagerung
- 4 °C
- Informationen zur Lagerung
- Store kit at 2-8° C prior to opening. See kit insert for complete instructions.
- Haltbarkeit
- See kit insert for complete instructions.
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- Hintergrund
- OxPhos™ is a medium based metabolic probe that can measure glutathione recycling capacity/glutathione function and glucose dependent antioxidant capacity of cells in tissue culture and whole blood. Oxphos™ is based on the ability of mammalian cells to rapidly and efficiently convert hydroxyethyl disulfide (HEDS) into mercaptoethanol (ME) through a bioreduction mechanism. Bioconversion of HEDS to ME relies on the activity of the oxidative pentose phosphate cycle (OPPC). OxPhos™ measures ME in the extracellular medium without the need for cellular lysis and extraction methods. Cell media is used in the assay, avoiding the need to lyse cells and thereby saving time and cost while preserving the ability to perform other cellular tests in the same culture system. OxPhos™ may have multiple applications in aging, oxidative stress, antioxidant screening, chemotherapy response and toxicology. Synonyms: Glutathione recycling, glutathione function, and glucose dependent antioxidant capacity assay, cell viability, cell survival cellular viability, cellular survival, antioxidant assay
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