PTX3 ELISA Kit

Produktnummer ABIN1672820

Produktdetails PTX3 ELISA Kit

Target: PTX3 (Pentraxin 3 (PTX3))

Bindungsspezifität: AA 18-381

Reaktivität: Maus

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 156-10000 pg/mL

Untere Nachweisgrenze: 156 pg/mL

Applikation: ELISA

Verwendungszweck: Sandwich High Sensitivity ELISA kit for Quantitative Detection of Mouse PTX3/Pentraxin 3

Marke: PicoKine™

Proben: Cell Culture Supernatant, Serum, Plasma (EDTA)

Analytische Methode: Quantitative

Spezifität: Expression system for standard: NSO
Immunogen sequence: E18-S381

Kreuzreaktivität (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensitivität: <10pg/mL

Benötigtes Material: Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl

Immunogen: Expression system for standard: NSO
Immunogen sequence: E18-S381

Anwendungsinformationen

Applikationshinweise: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Plattentyp: Pre-coated

Protokoll: mouse PTX3 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for PTX3 has been precoated onto 96-well plates. Standards(NSO, E18-S381) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for PTX3 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse PTX3 amount of sample captured in plate.

Testdurchführung:

Aliquot 0.1 mL per well of the 10000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 313pg/mL, 156pg/mL mouse PTX3 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum or plasma(EDTA) to each empty well. See "Sample Dilution Guideline" above for details. We recommend that each mouse PTX3 standard solution and each sample is measured in duplicate.

Testpräzision:

• Sample 1: n=16, Mean(pg/ml): 1274, Standard deviation: 56.1, CV(%): 4.4
• Sample 2: n=16, Mean(pg/ml): 2937, Standard deviation: 164.5, CV(%): 5.6
• Sample 3: n=16, Mean(pg/ml): 5436, Standard deviation: 342.5, CV(%): 6.3,
• Sample 1: n=24, Mean(pg/ml): 1435, Standard deviation: 83.23, CV(%): 5.8
• Sample 2: n=24, Mean(pg/ml): 3356, Standard deviation: 245, CV(%): 7.3
• Sample 3: n=24, Mean(pg/ml): 5628, Standard deviation: 382.7, CV(%): 6.8

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: Avoid multiple freeze-thaw cycles.

Lagerung: -20 °C,4 °C

Informationen zur Lagerung: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles

Haltbarkeit: 12 months

Details zu PTX3

Target: PTX3 (Pentraxin 3 (PTX3))

Andere Bezeichnung: PTX3 (PTX3 Produkte)

Synonyme: fb05a06 ELISA Kit, wu:fb05a06 ELISA Kit, TNFAIP5 ELISA Kit, TSG-14 ELISA Kit, PPTX ELISA Kit, AI607804 ELISA Kit, si:ch211-272f3.3 ELISA Kit, pentraxin 3 ELISA Kit, pentraxin related gene ELISA Kit, si:ch211-272f3.3 ELISA Kit, LOC100230343 ELISA Kit, PTX3 ELISA Kit, Ptx3 ELISA Kit

Hintergrund:

Protein Function: Plays a role in the regulation of innate resistance to pathogens, inflammatory reactions, possibly clearance of self- components and female fertility. .

Background: PTX3(Pentraxin 3) is a member of the pentraxin superfamily. This super family characterized by cyclic multimeric structure. The PTX3 gene is mapped to 3q25.32. The predicted 381-amino acid PTX3 protein has homology to the pentraxin protein family. Significant levels of PTX3 were detected in plasma of neutropenic patients with systemic A. PTX3 is effective in preventing CMV infection and reactivation, as well as subsequent Aspergillus infection. PTX3 activates the classical pathway of complementactivation and facilitates pathogen recognition by macrophages and DCs.

Synonyms: Pentraxin-related protein PTX3,Pentaxin-related protein PTX3,Tumor necrosis factor-inducible gene 14 protein,TSG-14,Ptx3,Tsg14,

Full Gene Name: Pentraxin-related protein PTX3

Cellular Localisation: Secreted.

Gen-ID: 19288

UniProt: P48759