CXCL5 ELISA Kit

Produktnummer ABIN1672835

Produktdetails CXCL5 ELISA Kit

Target: CXCL5 (Chemokine (C-X-C Motif) Ligand 5 (CXCL5))

Bindungsspezifität: AA 45-118

Reaktivität: Maus

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 15.6-1000 pg/mL

Untere Nachweisgrenze: 15.6 pg/mL

Applikation: ELISA

Verwendungszweck: Sandwich High Sensitivity ELISA kit for Quantitative Detection of Mouse CXCL5/ENA-78

Marke: PicoKine™

Proben: Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA), Plasma (citrate)

Analytische Methode: Quantitative

Spezifität: Expression system for standard: E.coli
Immunogen sequence: V45-A118

Kreuzreaktivität (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensitivität: <10pg/mL

Benötigtes Material: Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl

Immunogen: Expression system for standard: E.coli
Immunogen sequence: V45-A118

Anwendungsinformationen

Applikationshinweise: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Plattentyp: Pre-coated

Protokoll: mouse CXCL5 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for CXCL5 has been precoated onto 96-well plates. Standards(E.coli, V45-A118) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CXCL5 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse CXCL5 amount of sample captured in plate.

Testdurchführung:

Aliquot 0.1 mL per well of the 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL, 15.6pg/mL mouse CXCL5 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum or plasma(heparin, EDTA, citrate) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each mouse CXCL5 standard solution and each sample be measured in duplicate.

Testpräzision:

• Sample 1: n=16, Mean(pg/ml): 89, Standard deviation: 4.54, CV(%): 5.1
• Sample 2: n=16, Mean(pg/ml): 238, Standard deviation: 13.3, CV(%): 5.6
• Sample 3: n=16, Mean(pg/ml): 496, Standard deviation: 18.9, CV(%): 3.8,
• Sample 1: n=24, Mean(pg/ml): 103, Standard deviation: 7.42, CV(%): 7.2
• Sample 2: n=24, Mean(pg/ml): 276, Standard deviation: 20.7, CV(%): 7.5
• Sample 3: n=24, Mean(pg/ml): 513, Standard deviation: 32.8, CV(%): 6.4

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: Avoid multiple freeze-thaw cycles.

Lagerung: -20 °C,4 °C

Informationen zur Lagerung: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles

Haltbarkeit: 12 months

Details zu CXCL5

Target: CXCL5 (Chemokine (C-X-C Motif) Ligand 5 (CXCL5))

Andere Bezeichnung: CXCL5 (CXCL5 Produkte)

Synonyme: ENA-78 ELISA Kit, SCYB5 ELISA Kit, Cxcl6 ELISA Kit, CXCL5 ELISA Kit, GCP-2 ELISA Kit, GCP2 ELISA Kit, SCYB6 ELISA Kit, AMCF-II ELISA Kit, LIX ELISA Kit, Scyb5 ELISA Kit, Scyb6 ELISA Kit, C-X-C motif chemokine ligand 5 ELISA Kit, C-X-C motif chemokine ligand 6 ELISA Kit, chemokine (C-X-C motif) ligand 5 ELISA Kit, C-X-C motif chemokine 5 ELISA Kit, CXCL5 ELISA Kit, Cxcl6 ELISA Kit, LOC701946 ELISA Kit, LOC703222 ELISA Kit, Cxcl5 ELISA Kit

Hintergrund:

Protein Function: May participate in the recruitment of inflammatory cells by injured or infected tissue. GCP-2(1-78) and, more potent, GCP- 2(9-78) attract neutrophils and are involved in neutrophil activation. .

Background: C-X-C motif chemokine 5 is a protein that in humans encoded by the CXCL5 gene. The protein encoded by this gene, CXCL5 is a small cytokine belonging to the CXC chemokine family that is also known as epithelial-derived neutrophil-activating peptide 78(ENA-78). It is produced following stimulation of cells with the inflammatory cytokines interleukin-1or tumor necrosis factor-alpha. Expression of CXCL5 has also been observed in eosinophils, and can be inhibited with the type II interferon IFN-gamma. This chemokine stimulates the chemotaxis of neutrophils possessing angiogenic properties. It elicits these effects by interacting with the cell surface chemokine receptor CXCR2. The gene for CXCL5 is encoded on four exons and is located on humanchromosome 4 amongst several other CXC chemokine genes. CXCL5 has been implicated in connective tissue remodeling. CXCL5 plays a role in reducing sensitivity to sunburn pain in some subjects, and is a potential target which can be utilized to understand more about pain in other inflammatory conditions like arthritis and cystitis.

Synonyms: C-X-C motif chemokine 5,Cytokine LIX,Small-inducible cytokine B5,GCP-2(1-78),GCP-2(9-78),Cxcl5,Scyb5,

Full Gene Name: C-X-C motif chemokine 5

Cellular Localisation: Secreted.

Gen-ID: 20311

UniProt: P50228

Pathways: Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity