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IGFBP6 ELISA Kit

IGFBP6 Reaktivität: Human AA 25-240 Colorimetric Sandwich ELISA 62.5-4000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
Produktnummer ABIN1672866
  • Target Alle IGFBP6 ELISA Kits anzeigen
    IGFBP6 (Insulin-Like Growth Factor Binding Protein 6 (IGFBP6))
    Bindungsspezifität
    AA 25-240
    Reaktivität
    • 6
    • 5
    • 3
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Human
    Nachweismethode
    Colorimetric
    Methodentyp
    Sandwich ELISA
    Detektionsbereich
    62.5-4000 pg/mL
    Untere Nachweisgrenze
    62.5 pg/mL
    Applikation
    ELISA
    Verwendungszweck
    Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human IGFBP6
    Marke
    PicoKine™
    Proben
    Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)
    Analytische Methode
    Quantitative
    Spezifität
    Expression system for standard: NSO
    Immunogen sequence: A25-G240
    Kreuzreaktivität (Details)
    There is no detectable cross-reactivity with other relevant proteins.
    Sensitivität
    <10pg/mL
    Benötigtes Material
    Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
    Immunogen
    Expression system for standard: NSO
    Immunogen sequence: A25-G240
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  • Applikationshinweise
    Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
    Kommentare

    Sequence similarities: Contains 1 IGFBP N-terminal domain.

    Plattentyp
    Pre-coated
    Protokoll
    human IGFBP6 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for IGFBP6 has been precoated onto 96-well plates. Standards(NSO, A25-G240) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for IGFBP6 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human IGFBP6 amount of sample captured in plate.
    Testdurchführung

    Aliquot 0.1 mL per well of the 4000pg/mL, 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL human IGFBP6 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human IGFBP6 standard solution and each sample be measured in duplicate.

    Testpräzision
    • Sample 1: n=16, Mean(pg/ml): 551, Standard deviation: 21.5, CV(%): 3.9
    • Sample 2: n=16, Mean(pg/ml): 1557, Standard deviation: 63.84, CV(%): 4.1
    • Sample 3: n=16, Mean(pg/ml): 2736, Standard deviation: 128.6, CV(%): 4.7,
    • Sample 1: n=24, Mean(pg/ml): 489, Standard deviation: 27.87, CV(%): 5.7
    • Sample 2: n=24, Mean(pg/ml): 1635, Standard deviation: 101.37, CV(%): 6.2
    • Sample 3: n=24, Mean(pg/ml): 2878, Standard deviation: 184.2, CV(%): 6.4
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Handhabung
    Avoid multiple freeze-thaw cycles.
    Lagerung
    -20 °C,4 °C
    Informationen zur Lagerung
    Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
    Haltbarkeit
    12 months
  • Target Alle IGFBP6 ELISA Kits anzeigen
    IGFBP6 (Insulin-Like Growth Factor Binding Protein 6 (IGFBP6))
    Andere Bezeichnung
    IGFBP6 (IGFBP6 Produkte)
    Synonyme
    IBP6 ELISA Kit, IGFBP-6 ELISA Kit, IGF-BP6 ELISA Kit, RBP6A ELISA Kit, IGFBP6 ELISA Kit, igfbp6 ELISA Kit, insulin like growth factor binding protein 6 ELISA Kit, insulin-like growth factor binding protein 6 ELISA Kit, IGFBP6 ELISA Kit, Igfbp6 ELISA Kit, IGFBP-6 ELISA Kit, igfbp6 ELISA Kit
    Hintergrund

    Protein Function: IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors.

    Background: Insulin-like growth factor-binding protein 6(IGFBP6) is a protein that in humans is encoded by the IGFBP6 gene. The IGFBP6 gene is mapped to chromosome 12. In all, it has contained 216 amino acid protein with a calculated molecular mass of 22,847, The human keratinocyte cell line HaCat secretes IGFBP6 as an autocrine growth inhibitor. Recombinant IGFBP6 was also shown to inhibit growth of HaCat cells and other keratinocyte cell lines.

    Synonyms: Insulin-like growth factor-binding protein 6,IBP-6,IGF-binding protein 6,IGFBP-6,IGFBP6,IBP6,

    Full Gene Name: Insulin-like growth factor-binding protein 6

    Cellular Localisation: Secreted.
    Gen-ID
    3489
    UniProt
    P24592
    Pathways
    WNT Signalweg, Myometrial Relaxation and Contraction
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