Telefon:
+49 (0)241 95 163 153
Fax:
+49 (0)241 95 163 155
E-Mail:
orders@antikoerper-online.de

IL17RC ELISA Kit

IL17RC Reaktivität: Human AA 21-454 Colorimetric Sandwich ELISA 250-16000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
Produktnummer ABIN1672882
  • Target Alle IL17RC ELISA Kits anzeigen
    IL17RC (Interleukin 17 Receptor C (IL17RC))
    Bindungsspezifität
    AA 21-454
    Reaktivität
    • 4
    • 4
    Human
    Nachweismethode
    Colorimetric
    Methodentyp
    Sandwich ELISA
    Detektionsbereich
    250-16000 pg/mL
    Untere Nachweisgrenze
    250 pg/mL
    Applikation
    ELISA
    Verwendungszweck
    Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human IL-17RC
    Marke
    PicoKine™
    Proben
    Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)
    Analytische Methode
    Quantitative
    Spezifität
    Expression system for standard: NSO
    Immunogen sequence: L21-A454
    Kreuzreaktivität (Details)
    There is no detectable cross-reactivity with other relevant proteins.
    Sensitivität
    <15pg/mL
    Benötigtes Material
    Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
    Immunogen
    Expression system for standard: NSO
    Immunogen sequence: L21-A454
    Top Product
    Discover our top product IL17RC ELISA Kit
  • Applikationshinweise
    Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
    Kommentare

    Tissue Specificity: Expressed in brain, cartilage, colon, heart, intestine, kidney, liver, lung, muscle, placenta, and prostate. Low expression in thymus and leukocytes. Expressed (at protein level) in prostate and prostate cancer, skeletal muscle, kidney and placenta. .

    Plattentyp
    Pre-coated
    Protokoll
    human IL-17RC ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for IL-17RC has been precoated onto 96-well plates. Standards(NSO, L21-A454) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for IL-17RC is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human IL-17RC amount of sample captured in plate.
    Testdurchführung

    Aliquot 0.1 mL per well of the 16000pg/mL, 8000pg/mL, 4000pg/mL, 2000pg/mL, 1000pg/mL, 500pg/mL, 250pg/mL human IL-17RC standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human IL-17RC standard solution and each sample be measured in duplicate.

    Testpräzision
    • Sample 1: n=16, Mean(ng/ml): 1.8, Standard deviation: 0.101, CV(%): 5.6
    • Sample 2: n=16, Mean(ng/ml): 5, Standard deviation: 0.32, CV(%): 6.4
    • Sample 3: n=16, Mean(ng/ml): 9.7, Standard deviation: 0.427, CV(%): 4.4,
    • Sample 1: n=24, Mean(ng/ml): 2.2, Standard deviation: 0.132, CV(%): 6
    • Sample 2: n=24, Mean(ng/ml): 6.2, Standard deviation: 0.428, CV(%): 6.9
    • Sample 3: n=24, Mean(ng/ml): 10.3, Standard deviation: 0.546, CV(%): 5.3
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Handhabung
    Avoid multiple freeze-thaw cycles.
    Lagerung
    -20 °C,4 °C
    Informationen zur Lagerung
    Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
    Haltbarkeit
    12 months
  • Target Alle IL17RC ELISA Kits anzeigen
    IL17RC (Interleukin 17 Receptor C (IL17RC))
    Andere Bezeichnung
    IL17RC (IL17RC Produkte)
    Synonyme
    IL17-RL ELISA Kit, IL17RL ELISA Kit, 1110025H02Rik ELISA Kit, Gm19850 ELISA Kit, IL-17RC ELISA Kit, IL17-RC ELISA Kit, Il17rl ELISA Kit, interleukin 17 receptor C ELISA Kit, Il17rc ELISA Kit, IL17RC ELISA Kit
    Hintergrund

    Background: Interleukin-17 receptor C is a protein that in humans is encoded by the IL17RC gene. By genomic sequence analysis, IL17RC gene was mapped to chromosome 3p25.3-p24.1. This gene encodes a single-pass transmembrane protein that shares limited similarity with the interleukin-17 receptor. Multiple alternatively spliced transcript variants encoding different isoforms have been detected for this gene, but the full-length nature of only three have been determined to date. The biologic activity of IL-17 is dependent on a complex composed of IL-17RA and IL-17RC, suggesting a new paradigm for understanding the interactions between the expanded family of IL-17 ligands and their receptors.

    Synonyms: Interleukin-17 receptor C,IL-17 receptor C,IL-17RC,Interleukin-17 receptor homolog,IL17Rhom,Interleukin-17 receptor-like protein,IL-17RL,ZcytoR14,IL17RC,UNQ6118/PRO20040/PRO38901,

    Full Gene Name: Interleukin-17 receptor C

    Cellular Localisation: Cell membrane, Single-pass type I membrane protein. Soluble isoforms may be produced.
    Gen-ID
    84818
    UniProt
    Q8NAC3
Sie sind hier:
Kundenservice