TNFRSF4 ELISA Kit
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- Target Alle TNFRSF4 ELISA Kits anzeigen
- TNFRSF4 (Tumor Necrosis Factor Receptor Superfamily, Member 4 (TNFRSF4))
- Bindungsspezifität
- AA 20-211
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Reaktivität
- Maus
- Nachweismethode
- Colorimetric
- Methodentyp
- Sandwich ELISA
- Detektionsbereich
- 31.2-2000 pg/mL
- Untere Nachweisgrenze
- 31.2 pg/mL
- Applikation
- ELISA
- Verwendungszweck
- Sandwich High Sensitivity ELISA kit for Quantitative Detection of Mouse TNFRSF4/OX40
- Marke
- PicoKine™
- Proben
- Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)
- Analytische Methode
- Quantitative
- Spezifität
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Expression system for standard: NSO
Immunogen sequence: V20-P211 - Kreuzreaktivität (Details)
- There is no detectable cross-reactivity with other relevant proteins.
- Sensitivität
- <10pg/mL
- Benötigtes Material
- Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
- Immunogen
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Expression system for standard: NSO
Immunogen sequence: V20-P211 - Featured
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- Applikationshinweise
- Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
- Plattentyp
- Pre-coated
- Protokoll
- mouse TNFRSF4 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for TNFRSF4 has been precoated onto 96-well plates. Standards(NSO, V20-P211) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for TNFRSF4 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse TNFRSF4 amount of sample captured in plate.
- Testdurchführung
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Aliquot 0.1 mL per well of the 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL mouse TNFRSF4 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each mouse TNFRSF4 standard solution and each sample be measured in duplicate.
- Testpräzision
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- Sample 1: n=16, Mean(pg/ml): 224, Standard deviation: 7.62, CV(%): 3.4
- Sample 2: n=16, Mean(pg/ml): 732, Standard deviation: 32.94, CV(%): 4.5
- Sample 3: n=16, Mean(pg/ml): 1463, Standard deviation: 83.4, CV(%): 5.7,
- Sample 1: n=24, Mean(pg/ml): 364, Standard deviation: 17.5, CV(%): 4.8
- Sample 2: n=24, Mean(pg/ml): 885, Standard deviation: 49.56, CV(%): 5.6
- Sample 3: n=24, Mean(pg/ml): 1376, Standard deviation: 95, CV(%): 6.9
- Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Handhabung
- Avoid multiple freeze-thaw cycles.
- Lagerung
- -20 °C,4 °C
- Informationen zur Lagerung
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
- Haltbarkeit
- 12 months
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- Target Alle TNFRSF4 ELISA Kits anzeigen
- TNFRSF4 (Tumor Necrosis Factor Receptor Superfamily, Member 4 (TNFRSF4))
- Andere Bezeichnung
- TNFRSF4 (TNFRSF4 Produkte)
- Synonyme
- TNFRSF4 ELISA Kit, ACT35 ELISA Kit, CD134 ELISA Kit, Ly-70 ELISA Kit, Ox40 ELISA Kit, TXGP1L ELISA Kit, Txgp1 ELISA Kit, OX40 ELISA Kit, TNF receptor superfamily member 4 ELISA Kit, tumor necrosis factor receptor superfamily, member 4 ELISA Kit, TNFRSF4 ELISA Kit, Tnfrsf4 ELISA Kit
- Hintergrund
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Protein Function: Receptor for TNFSF4/OX40L/GP34. Is a costimulatory molecule implicated in long-term T-cell immunity (By similarity). .
Background: Tumor necrosis factor receptor superfamily, member4, also known as ACT35 or CD134 is a cell surface glycoprotein that was discovered through the production of a monoclonal antibody raised against the HUT-102 cell line. It belongs to the tumor necrosis factor receptor superfamily. CD134 was mapped to 1p36 by fluorescence in situ hybridization. CD134 is the primary receptor for feline immunodeficiency virus. CD134 expression can promote viral binding and renders cells permissive for viral entry, productive infection, and syncytium formation. Stimulating the receptor can improve the response to a powerful virus vector and may be useful in vaccine development.
Synonyms: Tumor necrosis factor receptor superfamily member 4,OX40 antigen,OX40L receptor,CD134,Tnfrsf4,Ox40, Txgp1,
Full Gene Name: Tumor necrosis factor receptor superfamily member 4
Cellular Localisation: Membrane, Single-pass type I membrane protein. - Gen-ID
- 22163
- UniProt
- P47741
- Pathways
- Production of Molecular Mediator of Immune Response, Cancer Immune Checkpoints
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