CCL6 ELISA Kit

Produktnummer ABIN1706076

Produktdetails CCL6 ELISA Kit

Target: CCL6 (Chemokine (C-C Motif) Ligand 6 (CCL6))

Bindungsspezifität: AA 22-116

Reaktivität: Maus

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 15.6-1000 pg/mL

Untere Nachweisgrenze: 15.6 pg/mL

Applikation: ELISA

Verwendungszweck: Sandwich High Sensitivity ELISA kit for Quantitative Detection of Mouse CCL6/C10

Marke: PicoKine™

Proben: Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)

Analytische Methode: Quantitative

Spezifität: Expression system for standard: E.coli
Immunogen sequence: G22-A116

Kreuzreaktivität (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensitivität: <10pg/mL

Benötigtes Material: Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl

Immunogen: Expression system for standard: E.coli
Immunogen sequence: G22-A116

Anwendungsinformationen

Applikationshinweise: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Kommentare:

Tissue Specificity: Expressed in myelopoietic bone marrow cultures stimulated by GM-CSF.

Plattentyp: Pre-coated

Protokoll: mouse CCL6 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for CCL6 has been precoated onto 96-well plates. Standards(E.coli, G22-A116) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CCL6 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse CCL6 amount of sample captured in plate.

Testdurchführung:

Aliquot 0.1 mL per well of the 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.3pg/mL, 15.6pg/mL mouse CCL6 standard solutions into the pre-coated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. We recommend that each mouse CCL6 standard solution and each sample is measured in duplicate.

Testpräzision:

• Sample 1: n=16, Mean(pg/ml): 43, Standard deviation: 2.4, CV(%): 5.6
• Sample 2: n=16, Mean(pg/ml): 159, Standard deviation: 7.5, CV(%): 4.7
• Sample 3: n=16, Mean(pg/ml): 630, Standard deviation: 21.4, CV(%): 3.4,
• Sample 1: n=24, Mean(pg/ml): 44, Standard deviation: 3.6, CV(%): 8.2
• Sample 2: n=24, Mean(pg/ml): 166, Standard deviation: 12.3, CV(%): 7.4
• Sample 3: n=24, Mean(pg/ml): 621, Standard deviation: 44.1, CV(%): 7.1

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: Avoid multiple freeze-thaw cycles.

Lagerung: -20 °C,4 °C

Informationen zur Lagerung: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles

Haltbarkeit: 12 months

Details zu CCL6

Target: CCL6 (Chemokine (C-C Motif) Ligand 6 (CCL6))

Andere Bezeichnung: Ccl6 (CCL6 Produkte)

Synonyme: MRP-1 ELISA Kit, Scya6 ELISA Kit, c10 ELISA Kit, Abcc1a ELISA Kit, Avcc1a ELISA Kit, Mrp ELISA Kit, Mrp1 ELISA Kit, Mrp-1 ELISA Kit, Scay6 ELISA Kit, chemokine (C-C motif) ligand 6 ELISA Kit, ATP binding cassette subfamily C member 1 ELISA Kit, Ccl6 ELISA Kit, Abcc1 ELISA Kit

Hintergrund:

Protein Function: CCL6(22-95) and CCL6(23-95) are potent chemoattractants. .

Background: Chemokine(C-C motif) ligand 6(CCL6) is a small cytokine belonging to the CC chemokine family that has only been identified in rodents.In mice, CCL6 is expressed in cells from neutrophil and macrophage lineages, and can be greatly induced under conditions suitable for myeloid cell differentiation. It is highly expressed in bone marrow cultures that have been stimulated with the cytokine GM-CSF. Some low levels of gene expression also occur in certain cell lines of myeloid origin(e.g. the immature myeloid cell lines DA3 and 32D cl3, and the macrophage cell line P388D) that can also be greatly induced in culture with GM-CSF. However, in activated T cell lines, expression of CCL6 is greatly reduced. CCL6 can also be induced in the mouse lung by the cytokine interleukin 13. Mouse CCL6 is located on chromosome 11. The cell surface receptor for CCL6 is believed to be the chemokine receptor CCR1.

Synonyms: C-C motif chemokine 6,Protein C10,Small-inducible cytokine A6,CCL6(22-95),CCL6(23-95),Ccl6,C10, Scya6,

Full Gene Name: C-C motif chemokine 6

Cellular Localisation: Secreted.

Gen-ID: 20305

UniProt: P27784