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HABA Biotin Quantitation Kit

EAA Colorimetric
Produktnummer ABIN1882419
  • Target Alle Biotin Produkte
    Biotin
    Nachweismethode
    Colorimetric
    Konjugat
    Biotin
    Applikation
    Enzyme Activity Assay (EAA)
    Produktmerkmale
    The HABA Biotin Quantitation Kit provides a convenient method for estimating the molar ratio of biotin to protein in biotin-protein conjugates or for quantitating biotin concentration in a solution. The assay employs HABA (4'-hydroxyazobenzene-2-carboxylic acid), a reagent that shows dramatic spectral changes when bound to avidin. Biotin easily displaces HABA from the HABA/Avidin complex, resulting in a decrease of absorption at 500 nm. The kit is best used to determine biotin concentration in the range from 2 to 16 µM. The assay can be performed in a cuvette or microplate format.
  • Kommentare

    Enzymatic Activity Assay kit

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Lagerung
    4 °C
    Informationen zur Lagerung
    Store Component A at -20 °C. Store all other components at 4 °C
  • Target
    Biotin
    Abstract
    Biotin Produkte
    Substanzklasse
    Chemical
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