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SensoLyte® 490 HIV Protease Assay Kit

FRET Reaktivität: Human Immunodeficiency Virus (HIV) Fluorometric
Produktnummer ABIN1882480
  • Target Alle HIV Produkte
    HIV (Human Immunodeficiency Virus (HIV))
    Reaktivität
    • 1
    • 1
    • 1
    Human Immunodeficiency Virus (HIV)
    Nachweismethode
    Fluorometric
    Applikation
    Fluorescence Resonance Energy Transfer Microscopy (FRET)
    Marke
    SensoLyte®
    Produktmerkmale
    The SensoLyte® 490 HIV Protease Assay Kit uses an optimized FRET peptide substrate for a continuous measurement of HIV protease activities. This FRET-based fluorogenic substrate is derived from a natural processing site for HIV-1 PR. Incubation of the recombinant HIV-1 PR with this substrate results in specific cleavage and a time-dependent increase in fluorescence intensity that is linearly related to the extent of substrate hydrolysis. Because of its simplicity and precision in the determination of reaction rates required for kinetic analysis, this kit offers many advantages over the commonly used HPLC or electrophoresis-based assays for peptide substrate hydrolysis by retroviral PRs.
  • Kommentare

    FRET-based Assay Kit

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Handhabung
    Protect Components A and B from light.
    Lagerung
    -20 °C
  • Hallengärd, Haller, Petersson, Boberg, Maltais, Isaguliants, Wahren, Bråve: "Increased expression and immunogenicity of HIV-1 protease following inactivation of the enzymatic activity." in: Vaccine, Vol. 29, Issue 4, pp. 839-48, (2011) (PubMed).

    Frick, Ginzburg, Lam: "A method to simultaneously monitor hepatitis C virus NS3 helicase and protease activities." in: Methods in molecular biology (Clifton, N.J.), Vol. 587, pp. 223-33, (2010) (PubMed).

    Yu, Kabashima, Tang, Shibata, Kitazato, Kobayashi, Lee, Kai: "Selective and facile assay of human immunodeficiency virus protease activity by a novel fluorogenic reaction." in: Analytical biochemistry, Vol. 397, Issue 2, pp. 197-201, (2010) (PubMed).

  • Target
    HIV (Human Immunodeficiency Virus (HIV))
    Abstract
    HIV Produkte
    Substanzklasse
    Virus, Virus
    Hintergrund
    HIV protease (PR) is identified as an important drug-screening target for the design of selective acquired immunodeficiency syndrome (AIDS) therapeutics.
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