SensoLyte® Plus 520 MMP-1 Assay Kit Fluorimetric and Enhanced Selectivity

Produktnummer ABIN1882511

Produktdetails

Target: MMP1 (Matrix Metallopeptidase 1 (Interstitial Collagenase) (MMP1))

Reaktivität: Human

Nachweismethode: Fluorometric

Applikation: Fluorescence Resonance Energy Transfer Microscopy (FRET)

Marke: SensoLyte®

Produktmerkmale: The SensoLyte® Plus 520 MMP-1 Assay Kit is designed for specifically detecting MMP-1 activity in biological samples which may contain multiple MMPs, such as culture medium, serum, plasma, synovial fluid, and tissue homogenate. A specific anti-MMP-1 monoclonal antibody is used in combination with an MMP fluorogenic substrate, 5-FAM/QXL™520 FRET peptide. The fluorescence signal is monitored at Ex/Em=490 nm/520 nm upon MMP-1-induced cleavage of the FRET substrate.

Anwendungsinformationen

Kommentare:

ELISA + FRET-based Assay Kit

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: -80 °C

Informationen zur Lagerung: Store all kit components, except Component B, at -20 °C. Store Component B at -80 °C. For convenience, Components D, G, H, I can be stored at RT.

Referenzen

Carrizzo, Lenzi, Procaccini, Damato, Biagioni, Ambrosio, Amodio, Remondelli, Del Giudice, Izzo, Malovini, Formisano, Gigantino, Madonna, Puca, Trimarco, Matarese, Fornai, Vecchione: "Pentraxin 3 Induces Vascular Endothelial Dysfunction Through a P-selectin/Matrix Metalloproteinase-1 Pathway." in: Circulation, Vol. 131, Issue 17, pp. 1495-505; discussion 1505, (2015) (PubMed).

Atay, Banskota, Crow, Sethi, Rink, Godwin: "Oncogenic KIT-containing exosomes increase gastrointestinal stromal tumor cell invasion." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 111, Issue 2, pp. 711-6, (2014) (PubMed).

Halvorsen, Dahl, Smedbakken, Singh, Michelsen, Skjelland, Krohg-Sørensen, Russell, Höpken, Lipp, Damås, Holm, Yndestad, Biessen, Aukrust: "Increased levels of CCR7 ligands in carotid atherosclerosis: different effects in macrophages and smooth muscle cells." in: Cardiovascular research, Vol. 102, Issue 1, pp. 148-56, (2014) (PubMed).

Mogami, Keller, Shi, Word: "Effect of thrombin on human amnion mesenchymal cells, mouse fetal membranes, and preterm birth." in: The Journal of biological chemistry, Vol. 289, Issue 19, pp. 13295-307, (2014) (PubMed).

Ozeki, Kawai, Yamaguchi, Hiyama, Kinoshita, Hase, Nakata, Kondo, Mogi, Nakamura: "IL-1?-induced matrix metalloproteinase-13 is activated by a disintegrin and metalloprotease-28-regulated proliferation of human osteoblast-like cells." in: Experimental cell research, Vol. 323, Issue 1, pp. 165-77, (2014) (PubMed).

Bogatkevich, Highland, Akter, Silver: "The PPAR? Agonist Rosiglitazone Is Antifibrotic for Scleroderma Lung Fibroblasts: Mechanisms of Action and Differential Racial Effects." in: Pulmonary medicine, Vol. 2012, pp. 545172, (2011) (PubMed).

Jian, Pelle, Yang, Pernodet, Maes, Huang: "Iron sensitizes keratinocytes and fibroblasts to UVA-mediated matrix metalloproteinase-1 through TNF-? and ERK activation." in: Experimental dermatology, Vol. 20, Issue 3, pp. 249-54, (2011) (PubMed).

Leong, Li, Gu, Sun, Zhou, Nasser, Laudier, Iqbal, Majeska, Schaffler, Goldring, Cardoso, Zaidi, Sun: "Physiological loading of joints prevents cartilage degradation through CITED2." in: FASEB journal : official publication of the Federation of American Societies for Experimental Biology, Vol. 25, Issue 1, pp. 182-91, (2011) (PubMed).

Park, Chung: "Epidermal growth factor-induced matrix metalloproteinase-1 expression is negatively regulated by p38 MAPK in human skin fibroblasts." in: Journal of dermatological science, Vol. 64, Issue 2, pp. 134-41, (2011) (PubMed).

Handy, Saxena, Fu, Lin, Mells, Gupta, Anania: "Adiponectin activation of AMPK disrupts leptin-mediated hepatic fibrosis via suppressors of cytokine signaling (SOCS-3)." in: Journal of cellular biochemistry, Vol. 110, Issue 5, pp. 1195-207, (2010) (PubMed).

Cowan, Mak, Colterjohn, Singh, Ghert: "Collagenase expression and activity in the stromal cells from giant cell tumour of bone." in: Bone, Vol. 44, Issue 5, pp. 865-71, (2009) (PubMed).

Moor, Vachon, Gould: "Proteolytic activity in wound fluids and tissues derived from chronic venous leg ulcers." in: Wound repair and regeneration : official publication of the Wound Healing Society [and] the European Tissue Repair Society, Vol. 17, Issue 6, pp. 832-9, (2009) (PubMed).

Shi, Ji, Qazi, Tarbell: "Interstitial flow promotes vascular fibroblast, myofibroblast, and smooth muscle cell motility in 3-D collagen I via upregulation of MMP-1." in: American journal of physiology. Heart and circulatory physiology, Vol. 297, Issue 4, pp. H1225-34, (2009) (PubMed).

Antigendetails

Target: MMP1 (Matrix Metallopeptidase 1 (Interstitial Collagenase) (MMP1))

Andere Bezeichnung: MMP-1 (MMP1 Produkte)

Synonyme: CLG Kit, CLGN Kit, Mmp1a Kit, CG4859 Kit, Dm1-MMP Kit, Dmel\\CG4859 Kit, MMP-1 Kit, MMP1 Kit, Mmp 1 Kit, dMMP1 Kit, dm1-MMP Kit, dmmp1 Kit, l(2)k04809 Kit, mmp1 Kit, Mmp1 Kit, Mcol-A Kit, Mcola Kit, col4 Kit, mmp18 Kit, Clgn Kit, matrix metallopeptidase 1 Kit, Matrix metalloproteinase 1 Kit, matrix metalloproteinase 1 Kit, matrix metallopeptidase 1 (interstitial collagenase) Kit, matrix metallopeptidase 1a (interstitial collagenase) Kit, matrix metallopeptidase 1 S homeolog Kit, interstitial collagenase Kit, matrix metallopeptidase 8 L homeolog Kit, MMP1 Kit, Mmp1 Kit, RB11133 Kit, Mmp1a Kit, mmp1.S Kit, LOC100727966 Kit, mmp8.L Kit