SensoLyte® 520 Neprilysin Activity Assay Kit

Produktnummer ABIN1882538

Produktdetails

Target: MME (Membrane Metallo-Endopeptidase (MME))

Nachweismethode: Fluorometric

Applikation: Activation (Act), Fluorescence Resonance Energy Transfer Microscopy (FRET)

Marke: SensoLyte®

Produktmerkmale: The SensoLyte® 520 Neprilysin Assay Kit employs a novel internally quenched 5-FAM/QXL™ FRET substrate for the detection of neprilysin activity. The enzyme cleaves the FRET substrate into two separate fragments resulting in the release of 5-FAM fluorescence, which can be monitored at excitation /emission= 490/520 nm. The long wavelength fluorescence of 5-FAM is less interfered by the autofluorescence of components in biological samples and test compounds. The assay can detect as low as 0.78 ng/mL of active neprilysin.

Anwendungsinformationen

Kommentare:

FRET-based Assay Kit

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: Protect Components A abd B from light and moisture.

Lagerung: -80 °C

Informationen zur Lagerung: Store all kit Components at -20 °C, except Component C, which should be stored at -80 °C.

Antigendetails

Target: MME (Membrane Metallo-Endopeptidase (MME))

Andere Bezeichnung: Neprilysin (MME Produkte)

Substanzklasse: Chemical

Hintergrund: Neprilysin (NEP) is a transmembrane metallopeptidase normally expressed by a variety of tissues. It is also known as neutral endopeptidase, and enkephalinase. NEP cleaves peptides at the N-terminal side of hydrophobic amino acid residues and is responsible for the degradation and inactivation of a variety of physiological substrates. NEP is a major extracellular amyloid beta-peptide degrading enzyme in the brain and targeting NEP is considered a potential therapeutic strategy for the prevention and treatment of Alzheimer's disease. NEP has also been implicated in the pathogenesis of hypertension, diabetes, and cancer.