SensoLyte® AFC Plasmin Activity Assay Kit

Produktnummer ABIN1882541

Produktdetails

Target: Plasmin (Plasmin/fibrinolysin (PL/Fbn))

Nachweismethode: Fluorometric

Applikation: Activation (Act), Fluorescence Resonance Energy Transfer Microscopy (FRET)

Marke: SensoLyte®

Produktmerkmale: The SensoLyte® AFC Plasmin Activity Assay Kit provides a convenient assay for high throughput screening of plasmin inhibitors and inducers or for continuous assay of plasmin activity. The assay utilizes a fluorogenic peptide, which upon enzyme cleavage releases the AFC (7-amido-4-trifluoromethylcoumarin) fluorophore. The fluorescent signal can be measured at Ex/ Em =380 nm/500 nm. This assay can detect picogram level of plasmin activity.

Anwendungsinformationen

Kommentare:

FRET-based Assay Kit

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: -80 °C

Informationen zur Lagerung: Store all kit components at -20 °C, except for Component C. Store Component C at -80 °C. Protect Components A and B from light and moisture. Components D and F can be stored at room temperature for convenience.

Referenzen

Portelli, Siedlinski, Stewart, Postma, Nieuwenhuis, Vonk, Nurnberg, Altmuller, Moffatt, Wardlaw, Parker, Connolly, Koppelman, Sayers: "Genome-wide protein QTL mapping identifies human plasma kallikrein as a post-translational regulator of serum uPAR levels." in: FASEB journal : official publication of the Federation of American Societies for Experimental Biology, Vol. 28, Issue 2, pp. 923-34, (2014) (PubMed).

Park, Oh, Kim, Cho, Kim: "Identification of a specific haptoglobin C-terminal fragment in arthritic synovial fluid and its effect on interleukin-6 expression." in: Immunology, Vol. 140, Issue 1, pp. 133-41, (2013) (PubMed).

Stewart, Nijmeh, Brightling, Sayers: "uPAR regulates bronchial epithelial repair in vitro and is elevated in asthmatic epithelium." in: Thorax, Vol. 67, Issue 6, pp. 477-87, (2012) (PubMed).

Tudpor, Laínez, Kwakernaak, Kovalevskaya, Verkaart, van Genesen, van der Kemp, Navis, Bindels, Hoenderop: "Urinary plasmin inhibits TRPV5 in nephrotic-range proteinuria." in: Journal of the American Society of Nephrology : JASN, Vol. 23, Issue 11, pp. 1824-34, (2012) (PubMed).

Antigendetails

Target: Plasmin (Plasmin/fibrinolysin (PL/Fbn))

Andere Bezeichnung: Plasmin (Plasmin Produkte)

Synonyme: plasminogen Kit, PLG Kit

Hintergrund: Plasmin belongs to the family of serine proteases. It plays a key role in fibrinolysis by dissolving fibrin in blood clots. Besides fibrinolysis, plasmin is also involved in such physiological and pathological processes as wound healing, liver repair, and the maintenance of liver homeostasis.