SensoLyte® 520 Ratte Renin Assay Kit

Produktnummer ABIN1882548

Kurzübersicht für SensoLyte® 520 Ratte Renin Assay Kit (ABIN1882548)

Target: Renin (REN)

Reaktivität: Ratte

Nachweismethode: Fluorometric

Applikation: Fluorescence Resonance Energy Transfer Microscopy (FRET)

Produktdetails

Marke: SensoLyte®

Produktmerkmale: The SensoLyte® 520 Rat Renin Assay Kit provides a convenient assay for high throughput screening of renin inhibitors and for continuous assay of renin activity using a 5-FAM/QXL™520 fluorescence resonance energy transfer (FRET) peptide. In the FRET peptide the fluorescence of 5-FAM is quenched by QXL™520. Upon cleavage into two separate fragments by renin, the fluorescence of 5-FAM is recovered, and can be monitored at excitation/emission = 490/520 nm.

Anwendungsinformationen

Kommentare:

FRET-based Assay Kit

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: Protect Components A and B from light and moisture.

Lagerung: -80 °C

Informationen zur Lagerung: Store all kit components at -20 °C, except for Component C. Store Component C at -80 °C. Component D can be stored at room temperature for convenience

Referenzen

Matsushima, Furuuchi, Shirai, Nagai, Yokoyama, Nishida, Hirayama: "Effects of acute and chronic boysenberry intake on blood pressure and endothelial function in spontaneous hypertensive rats." in: Journal of nutritional science and vitaminology, Vol. 60, Issue 1, pp. 43-51, (2014) (PubMed).

Chelko, Schmiedt, Lewis, Lewis, Robertson: "A novel vascular clip design for the reliable induction of 2-kidney, 1-clip hypertension in the rat." in: Journal of applied physiology (Bethesda, Md. : 1985), Vol. 112, Issue 3, pp. 362-6, (2012) (PubMed).

Shah, Dhar, El Zahraa Mohammed, Habtesion, Davies, Jover-Cobos, Macnaughtan, Sharma, Olde Damink, Mookerjee, Jalan: "Prevention of acute kidney injury in a rodent model of cirrhosis following selective gut decontamination is associated with reduced renal TLR4 expression." in: Journal of hepatology, Vol. 56, Issue 5, pp. 1047-53, (2012) (PubMed).

Antigendetails

Target: Renin (REN)

Andere Bezeichnung: Renin

Hintergrund: Renin, a highly specific aspartyl protease, cleaves angiotensinogen, to yield angiotensin I, which is further converted into angiotensin II by ACE (Angiotensin Converting Enzyme). Angiotensin II constricts blood vessels leading to increased blood pressure. Discovery of new renin inhibitors for treatment of hypertension is a promising area of research.