SensoLyte® Thiol Quantitation Assay Kit
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- Target
- Thiol
- Nachweismethode
- Colorimetric
- Applikation
- Enzyme Activity Assay (EAA)
- Marke
- SensoLyte®
- Produktmerkmale
- The SensoLyte® Thiol Quantitation Assay Kit utilizes the widely used Elmann's reagent for colorimetric measurement of thiol concentrations. The sulfhydryl group of thiol reacts with Ellman's Reagent [5, 5'-dithiobis (2-nitrobenzoic acid)] and generates 2-nitro-5-thiobenzoic acid (TNB) producing a yellow color that can be detected at absorbance 420 nm. The intensity of the color produced is proportional to thiol concentration.
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- Kommentare
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Enzymatic Activity Assay kit
- Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Handhabung
- Protect Component A from light and moisture.
- Lagerung
- -20 °C
- Informationen zur Lagerung
- Store all kit components at -20 °C. Component C can be stored at room temperature for convenience.
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- Target
- Thiol
- Hintergrund
- Thiols, compounds that contain sulfhydryl group, are powerful reducing agents capable of acting as antioxidants, enzyme cofactors, and neuromodulators.
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