CCL3 ELISA Kit

Produktnummer ABIN1889299

Maus CCL3 ELISA-Kit Colorimetric-Assay zur Quantifizierung von Maus CCL3 und wurde in 2+ Publikationen erwähnt.

Kurzübersicht für CCL3 ELISA Kit (ABIN1889299)

Target: CCL3 (Chemokine (C-C Motif) Ligand 3 (CCL3))

Bindungsspezifität: AA 24-92

Reaktivität: Maus

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 7.8-500 pg/mL

Applikation: ELISA

Proben: Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA), Plasma (citrate)

Produktdetails CCL3 ELISA Kit

Untere Nachweisgrenze: 7.8 pg/mL

Verwendungszweck: Sandwich High Sensitivity ELISA kit for Quantitative Detection of Mouse CCL3/MIP1 alpha

Marke: PicoKine™

Analytische Methode: Quantitative

Spezifität: Expression system for standard: E.coli
Immunogen sequence: A24-A92

Kreuzreaktivität (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensitivität: <10pg/mL

Benötigtes Material: Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl

Immunogen: Expression system for standard: E.coli
Immunogen sequence: A24-A92

Anwendungsinformationen

Applikationshinweise: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Kommentare:

Tissue Specificity: Expressed in lung, spleen, and pancreas.

Plattentyp: Pre-coated

Protokoll: mouse CCL3 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for CCL3 has been precoated onto 96-well plates. Standards(E.coli, A24-A92) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CCL3 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse CCL3 amount of sample captured in plate.

Testdurchführung:

Aliquot 0.1 mL per well of the 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.3pg/mL, 15.6pg/mL, 7.8pg/mL mouse CCL3 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum or plasma(heparin, EDTA, citrate) to each empty well. See "Sample Dilution Guideline" above for details. We recommend that each mouse CCL3 standard solution and each sample is measured in duplicate.

Testpräzision:

• Sample 1: n=16, Mean(pg/ml): 102, Standard deviation: 3.88, CV(%): 3.8
• Sample 2: n=16, Mean(pg/ml): 234, Standard deviation: 9.6, CV(%): 4.1
• Sample 3: n=16, Mean(pg/ml): 421, Standard deviation: 22.3, CV(%): 5.3,
• Sample 1: n=24, Mean(pg/ml): 86, Standard deviation: 4.82, CV(%): 5.6
• Sample 2: n=24, Mean(pg/ml): 255, Standard deviation: 17, CV(%): 6.7
• Sample 3: n=24, Mean(pg/ml): 437, Standard deviation: 31, CV(%): 7.1

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: Avoid multiple freeze-thaw cycles.

Lagerung: -20 °C,4 °C

Informationen zur Lagerung: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles

Haltbarkeit: 12 months

Referenzen für CCL3 ELISA Kit (ABIN1889299)

Du, Chen, Wang, Sun: "Pathway analysis of global gene expression change in dendritic cells induced by the polysaccharide from the roots of Actinidia eriantha." in: Journal of ethnopharmacology, Vol. 214, pp. 141-152, (2018) (PubMed).

Aziz, Cui, Das, Brown, Ardell, Febbraio, Pluskota, Han, Wu, Ballantyne, Smith, Cathcart, Yakubenko: "The Upregulation of Integrin αDβ2 (CD11d/CD18) on Inflammatory Macrophages Promotes Macrophage Retention in Vascular Lesions and Development of Atherosclerosis." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 198, Issue 12, pp. 4855-4867, (2017) (PubMed).

Details zu CCL3

Target: CCL3 (Chemokine (C-C Motif) Ligand 3 (CCL3))

Andere Bezeichnung: CCL3

Hintergrund:

Protein Function: Monokine with inflammatory, pyrogenic and chemokinetic properties. Has a potent chemotactic activity for eosinophils. Binding to a high-affinity receptor activates calcium release in neutrophils.

Background: Chemokine(C-C motif) ligand 3(CCL3), so known as Macrophage inflammatory protein-1alpha(MIP-1 alpha), is a protein that in humans is encoded by the CCL3 gene. It is a cytokine which belongs to the CC chemokine family that is involved in the acute inflammatory state in the recruitment and activation of polymorphonuclear leukocytes. This gene is mapped to 17q12. CCL3 plays a role in inflammatory responses through binding to the receptors CCR1, CCR4 and CCR5. It has been found that the activation of CCR5 by CCL3 directly and independently activates a G-protein signaling pathway through GNAI2 and a tyrosine phosphorylation signaling pathway through JAK2.

Synonyms: C-C motif chemokine 3,Heparin-binding chemotaxis protein,L2G25B,Macrophage inflammatory protein 1-alpha,MIP-1-alpha,SIS-alpha,Small-inducible cytokine A3,TY-5,Ccl3,Mip1a, Scya3,

Full Gene Name: C-C motif chemokine 3

Cellular Localisation: Secreted.

Gen-ID: 20302

UniProt: P10855

Pathways: Cellular Response to Molecule of Bacterial Origin, Autophagie