CXCL7 ELISA Kit

Produktnummer ABIN1889316

Produktdetails CXCL7 ELISA Kit

Target: CXCL7 (PPBP) (Pro-Platelet Basic Protein (Chemokine (C-X-C Motif) Ligand 7) (PPBP))

Bindungsspezifität: AA 59-128

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 15.6-1000 pg/mL

Untere Nachweisgrenze: 15.6 pg/mL

Applikation: ELISA

Verwendungszweck: Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human CXCL7

Marke: PicoKine™

Proben: Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)

Analytische Methode: Quantitative

Spezifität: Expression system for standard: E.coli
Immunogen sequence: A59-D128

Kreuzreaktivität (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensitivität: <2pg/mL

Benötigtes Material: Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl

Immunogen: Expression system for standard: E.coli
Immunogen sequence: A59-D128

Anwendungsinformationen

Applikationshinweise: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Kommentare:

Sequence similarities: Belongs to the intercrine alpha (chemokine CxC) family.

Plattentyp: Pre-coated

Protokoll: human CXCL7 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for CXCL7 has been precoated onto 96-well plates. Standards(E.coli, A59-D128) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CXCL7 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human CXCL7 amount of sample captured in plate.

Testdurchführung:

Aliquot 0.1 mL per well of the 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL, 15.6pg/mL human CXCL7 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma (heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human CXCL7 standard solution and each sample be measured in duplicate.

Testpräzision:

• Sample 1: n=16, Mean(pg/ml): 85.6, Standard deviation: 3.85, CV(%): 4.5
• Sample 2: n=16, Mean(pg/ml): 227, Standard deviation: 12.7, CV(%): 5.6
• Sample 3: n=16, Mean(pg/ml): 519, Standard deviation: 30.1, CV(%): 5.8,
• Sample 1: n=24, Mean(pg/ml): 82.8, Standard deviation: 6.13, CV(%): 7.4
• Sample 2: n=24, Mean(pg/ml): 236, Standard deviation: 14.6, CV(%): 6.2
• Sample 3: n=24, Mean(pg/ml): 527, Standard deviation: 37.4, CV(%): 7.1

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: Avoid multiple freeze-thaw cycles.

Lagerung: -20 °C,4 °C

Informationen zur Lagerung: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles

Haltbarkeit: 12 months

Details zu CXCL7

Target: CXCL7 (PPBP) (Pro-Platelet Basic Protein (Chemokine (C-X-C Motif) Ligand 7) (PPBP))

Andere Bezeichnung: CXCL7 (PPBP Produkte)

Synonyme: 2400003M24Rik ELISA Kit, AI854500 ELISA Kit, CTAP3 ELISA Kit, CTAPIII ELISA Kit, Cxcl7 ELISA Kit, LA-PF4 ELISA Kit, LDGF ELISA Kit, MDGF ELISA Kit, NAP-2 ELISA Kit, NAP-2-L1 ELISA Kit, Scyb7 ELISA Kit, TGB ELISA Kit, TGB1 ELISA Kit, THBGB1 ELISA Kit, b-TG1 ELISA Kit, beta-TG ELISA Kit, B-TG1 ELISA Kit, Beta-TG ELISA Kit, CTAP-III ELISA Kit, CXCL7 ELISA Kit, PBP ELISA Kit, SCYB7 ELISA Kit, TC1 ELISA Kit, TC2 ELISA Kit, THBGB ELISA Kit, Nap-2 ELISA Kit, CTAPIII/NAP-2 ELISA Kit, Platelet basic protein ELISA Kit, pro-platelet basic protein ELISA Kit, scyb7 ELISA Kit, Ppbp ELISA Kit, PPBP ELISA Kit

Hintergrund:

Protein Function: LA-PF4 stimulates DNA synthesis, mitosis, glycolysis, intracellular cAMP accumulation, prostaglandin E2 secretion, and synthesis of hyaluronic acid and sulfated glycosaminoglycan. It also stimulates the formation and secretion of plasminogen activator by human synovial cells. NAP-2 is a ligand for CXCR1 and CXCR2, and NAP-2, NAP-2(73), NAP-2(74), NAP-2(1-66), and most potent NAP-2(1-63) are chemoattractants and activators for neutrophils. TC-1 and TC-2 are antibacterial proteins, in vitro released from activated platelet alpha-granules. CTAP-III(1-81) is more potent than CTAP-III desensitize chemokine-induced neutrophil activation. .

Background: Chemokine(C-X-C motif) ligand 7(CXCL7), also called PPBP or SCYB7, is a human gene. The encoded protein, Chemokine(C-X-C motif) ligand is a small cytokine belonging to the CXC chemokine family. CXCL7 is mapped to 4q13.3. It is a protein that is released in large amounts from platelets following their activation. CXCL7 is the precursor of the 2 platelet alpha-granule proteins, platelet basic protein(PBP) and connective tissue-activating peptide III(CTAP3). It stimulates various processes including mitogenesis, synthesis of extracellular matrix, glucose metabolism and synthesis of plasminogen activator. It also stimulates the formation and secretion of plasminogen activator by synovial cells.

Synonyms: Platelet basic protein,PBP,C-X-C motif chemokine 7,Leukocyte-derived growth factor,LDGF,Macrophage-derived growth factor,MDGF,Small-inducible cytokine B7,Connective tissue-activating peptide III,CTAP-III,LA-PF4,Low-affinity platelet factor IV,TC-2,Connective tissue-activating peptide III(1-81),CTAP-III(1-81),Beta-thromboglobulin,Beta-TG,Neutrophil-activating peptide 2(74),NAP-2(74),Neutrophil-activating peptide 2(73),NAP-2(73),Neutrophil-activating peptide 2,NAP-2,TC-1,Neutrophil-activating peptide 2(1-66),NAP-2(1-66),Neutrophil-activating peptide 2(1-63),NAP-2(1-63),PPBP,CTAP3, CXCL7, SCYB7, TGB1, THBGB1,

Full Gene Name: Platelet basic protein

Cellular Localisation: Secreted.

Gen-ID: 5473

UniProt: P02775