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Nidogen 1 ELISA Kit

NID1 Reaktivität: Human AA 29-1114 Colorimetric Sandwich ELISA 62.5-4000 pg/mL Cell Culture Supernatant, Serum
Produktnummer ABIN1889326
  • Target Alle Nidogen 1 (NID1) ELISA Kits anzeigen
    Nidogen 1 (NID1)
    Bindungsspezifität
    AA 29-1114
    Reaktivität
    • 4
    • 2
    • 1
    • 1
    Human
    Nachweismethode
    Colorimetric
    Methodentyp
    Sandwich ELISA
    Detektionsbereich
    62.5-4000 pg/mL
    Untere Nachweisgrenze
    62.5 pg/mL
    Applikation
    ELISA
    Verwendungszweck
    Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human Nidogen-1
    Marke
    PicoKine™
    Proben
    Cell Culture Supernatant, Serum
    Analytische Methode
    Quantitative
    Spezifität
    Expression system for standard: NSO
    Immunogen sequence: L29-K1114
    Kreuzreaktivität (Details)
    There is no detectable cross-reactivity with other relevant proteins.
    Sensitivität
    <10pg/mL
    Benötigtes Material
    Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
    Immunogen
    Expression system for standard: NSO
    Immunogen sequence: L29-K1114
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  • Applikationshinweise
    Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
    Kommentare

    Sequence similarities: Contains 6 EGF-like domains.

    Plattentyp
    Pre-coated
    Protokoll
    human Nidogen-1 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for Nidogen-1 has been precoated onto 96-well plates. Standards(NSO, L29-K1114) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Nidogen-1 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human Nidogen-1 amount of sample captured in plate.
    Testdurchführung

    Aliquot 0.1 mL per well of the 4000pg/mL, 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL human Nidogen-1 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates or serum to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human Nidogen-1 standard solution and each sample be measured in duplicate.

    Testpräzision
    • Sample 1: n=16, Mean(pg/ml): 322, Standard deviation: 14.17, CV(%): 4.4
    • Sample 2: n=16, Mean(pg/ml): 1438, Standard deviation: 74.8, CV(%): 5.2
    • Sample 3: n=16, Mean(pg/ml): 2638, Standard deviation: 166.2, CV(%): 6.3,
    • Sample 1: n=24, Mean(pg/ml): 547, Standard deviation: 31.18, CV(%): 5.7
    • Sample 2: n=24, Mean(pg/ml): 1729, Standard deviation: 105.5, CV(%): 6.1
    • Sample 3: n=24, Mean(pg/ml): 2875, Standard deviation: 186.9, CV(%): 6.5
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Handhabung
    Avoid multiple freeze-thaw cycles.
    Lagerung
    -20 °C,4 °C
    Informationen zur Lagerung
    Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
    Haltbarkeit
    12 months
  • Target Alle Nidogen 1 (NID1) ELISA Kits anzeigen
    Nidogen 1 (NID1)
    Andere Bezeichnung
    NID1 (NID1 Produkte)
    Synonyme
    NID1 ELISA Kit, nid1 ELISA Kit, im:7055206 ELISA Kit, im:7142300 ELISA Kit, mg:bb01h02 ELISA Kit, LOC100219409 ELISA Kit, A630025O17 ELISA Kit, Nid ELISA Kit, entactin ELISA Kit, entactin-1 ELISA Kit, nidogen-1 ELISA Kit, NID ELISA Kit, nidogen 1 ELISA Kit, nidogen 1a ELISA Kit, NID1 ELISA Kit, nid1a ELISA Kit, nid1 ELISA Kit, Nid1 ELISA Kit
    Hintergrund

    Protein Function: Sulfated glycoprotein widely distributed in basement membranes and tightly associated with laminin. Also binds to collagen IV and perlecan. It probably has a role in cell- extracellular matrix interactions.

    Background: Nidogen-1(NID-1), also known as Entactin, is a protein that in humans is encoded by the NID1 gene. It is a member of the nidogen family of basement membrane glycoproteins. This gene is mapped to 1q42.3. Nidogen-1 is a component of the basement membrane alongside other components such as collagen type IV, proteoglycans(heparan sulfate and glycosaminoglycans), laminin and fibronectin. The protein interacts with several other components of basement membranes. Structurally it(along with perlecan) connects the networks formed by collagens and laminins to each other. It may also play a role in cell interactions with the extracellular matrix. Nidogen-1 also can serve as a bridge between the 2 most abundant molecules in the basement membrane: type IV collagen and laminin.

    Synonyms: Nidogen-1,NID-1,Entactin,NID1,NID,

    Full Gene Name: Nidogen-1

    Cellular Localisation: Secreted, extracellular space, extracellular matrix, basement membrane.
    Gen-ID
    4811
    UniProt
    P14543
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