TGFB2 ELISA Kit

Produktnummer ABIN1889332

Produktdetails TGFB2 ELISA Kit

Target: TGFB2 (Transforming Growth Factor, beta 2 (TGFB2))

Bindungsspezifität: AA 303-414

Reaktivität: Maus

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 31.2-2000 pg/mL

Untere Nachweisgrenze: 31.2 pg/mL

Applikation: ELISA

Verwendungszweck: Sandwich High Sensitivity ELISA kit for Quantitative Detection of activated Mouse TGF-beta 2

Marke: PicoKine™

Proben: Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA), Plasma (citrate)

Analytische Methode: Quantitative

Spezifität: Expression system for standard: NSO
Immunogen sequence: A303-S414

Kreuzreaktivität (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensitivität: <10pg/mL

Benötigtes Material: Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl

Immunogen: Expression system for standard: NSO
Immunogen sequence: A303-S414

Anwendungsinformationen

Applikationshinweise: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Plattentyp: Pre-coated

Protokoll: mouse TGF-beta 2 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for TGF-beta 2 has been precoated onto 96-well plates. Standards(NSO, A303-S414) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for TGF-beta 2 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse TGF-beta 2 amount of sample captured in plate.

Testdurchführung:

Aliquot 0.1 mL per well of the 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL mouse TGF-beta 2 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of activated mouse cell culture supernates, serum or plasma(heparin, EDTA, citrate) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each mouse TGF-beta 2 standard solution and each sample be measured in duplicate.

Testpräzision:

• Sample 1: n=16, Mean(pg/ml): 242, Standard deviation: 10.9, CV(%): 4.5
• Sample 2: n=16, Mean(pg/ml): 524, Standard deviation: 28.3, CV(%): 5.4
• Sample 3: n=16, Mean(pg/ml): 1096, Standard deviation: 73.43, CV(%): 6.7,
• Sample 1: n=24, Mean(pg/ml): 253, Standard deviation: 13, CV(%): 5.1
• Sample 2: n=24, Mean(pg/ml): 487, Standard deviation: 35.55, CV(%): 7.3
• Sample 3: n=24, Mean(pg/ml): 1295, Standard deviation: 90.65, CV(%): 7

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: Avoid multiple freeze-thaw cycles.

Lagerung: -20 °C,4 °C

Informationen zur Lagerung: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles

Haltbarkeit: 12 months

Referenzen für TGFB2 ELISA Kit (ABIN1889332)

Li, Liu, Shen, Wang, Chen, Xu, Wen: "MicroRNA-26a modulates transforming growth factor beta-1-induced proliferation in human fetal lung fibroblasts." in: Biochemical and biophysical research communications, Vol. 454, Issue 4, pp. 512-517, (2014) (PubMed).

Details zu TGFB2

Target: TGFB2 (Transforming Growth Factor, beta 2 (TGFB2))

Andere Bezeichnung: TGFB2 (TGFB2 Produkte)

Synonyme: LDS4 ELISA Kit, TGF-beta2 ELISA Kit, tgf-beta2 ELISA Kit, tgfb2-A ELISA Kit, BB105277 ELISA Kit, Tgf-beta2 ELISA Kit, Tgfb-2 ELISA Kit, TGF-beta 2 ELISA Kit, Tgfbr2T ELISA Kit, TGFB2 ELISA Kit, TGFbeta2 ELISA Kit, MGF ELISA Kit, TGF-B2 ELISA Kit, transforming growth factor beta 2 ELISA Kit, transforming growth factor beta 2 L homeolog ELISA Kit, transforming growth factor, beta 2 ELISA Kit, transforming growth factor, beta receptor 2 ELISA Kit, TGFB2 ELISA Kit, tgfb2.L ELISA Kit, Tgfb2 ELISA Kit, Tgfbr2 ELISA Kit, tgfb2 ELISA Kit

Hintergrund:

Protein Function: TGF-beta 2 has suppressive effects on interleukin-2 dependent T-cell growth.

Background: Transforming growth factor-beta 2(TGF-beta 2) is a secreted protein known as a cytokine that performs many cellular functions and has a vital role during embryonic development. This gene is mapped to 1q41. It is an extracellular glycosylated protein. It is known to suppress the effects of interleukin dependent T-cell tumors. TGF-beta 2 is present at elevated levels in the aqueous humor of patients with primary open angle glaucoma(POAG). Studies have shown that TGF-beta 2 influences cultured trabecular meshwork cells, and it reduced outflow facility when perfused into cultured human anterior segments. In POAG, elevated expression of Gremlin by TM cells inhibited BMP4 antagonism of TGF-beta 2 and led to increased extracellular matrix deposition and elevated IOP.

Synonyms: Transforming growth factor beta-2,TGF-beta-2,Latency-associated peptide,LAP,Tgfb2,

Full Gene Name: Transforming growth factor beta-2

Cellular Localisation: Secreted.

Gen-ID: 21808

UniProt: P27090

Pathways: Cell-Cell Junction Organization, Production of Molecular Mediator of Immune Response, Protein targeting to Nucleus