N-Cadherin ELISA Kit

Produktnummer ABIN1889345

Produktdetails N-Cadherin ELISA Kit

Target: N-Cadherin (CDH2) (Cadherin 2 (CDH2))

Bindungsspezifität: AA 160-724

Reaktivität: Maus

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.78-50 ng/mL

Untere Nachweisgrenze: 0.78 ng/mL

Applikation: ELISA

Verwendungszweck: Sandwich High Sensitivity ELISA kit for Quantitative Detection of Mouse Cadherin-2/N-Cadherin

Marke: PicoKine™

Proben: Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)

Analytische Methode: Quantitative

Spezifität: Expression system for standard: NSO
Immunogen sequence: D160-A724

Kreuzreaktivität (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensitivität: <20pg/mL

Benötigtes Material: Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl

Immunogen: Expression system for standard: NSO
Immunogen sequence: D160-A724

Anwendungsinformationen

Applikationshinweise: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Plattentyp: Pre-coated

Protokoll: mouse Cadherin-2 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for Cadherin-2 has been precoated onto 96-well plates. Standards(NSO, D160-A724) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Cadherin-2 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse Cadherin-2 amount of sample captured in plate.

Testdurchführung:

Aliquot 0.1 mL per well of the 50 ng/mL, 25 ng/mL, 12.5 ng/mL, 6.25 ng/mL, 3.12 ng/mL, 1.56 ng/mL, 0.78 ng/mL mouse Cadherin-2 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. We recommend that each mouse Cadherin-2 standard solution and each sample is measured in duplicate.

Testpräzision:

• Sample 1: n=16, Mean(ng/ml): 14.5, Standard deviation: 0.609, CV(%): 4.2
• Sample 2: n=16, Mean(ng/ml): 31.8, Standard deviation: 1.24, CV(%): 3.9
• Sample 3: n=16, Mean(ng/ml): 41.2, Standard deviation: 2.11, CV(%): 5.1,
• Sample 1: n=24, Mean(ng/ml): 15.5, Standard deviation: 0.899, CV(%): 5.8
• Sample 2: n=24, Mean(ng/ml): 34.2, Standard deviation: 1.78, CV(%): 5.2
• Sample 3: n=24, Mean(ng/ml): 42.5, Standard deviation: 2.72, CV(%): 6.4

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: Avoid multiple freeze-thaw cycles.

Lagerung: -20 °C,4 °C

Informationen zur Lagerung: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles

Haltbarkeit: 12 months

Details zu N-Cadherin

Target: N-Cadherin (CDH2) (Cadherin 2 (CDH2))

Andere Bezeichnung: CDH2 (CDH2 Produkte)

Synonyme: CD325 ELISA Kit, CDHN ELISA Kit, CDw325 ELISA Kit, NCAD ELISA Kit, N-cadherin ELISA Kit, cadherin-2 ELISA Kit, cdhn ELISA Kit, ncad ELISA Kit, cdw325 ELISA Kit, CDH2 ELISA Kit, Cadherin-2 ELISA Kit, Ncad ELISA Kit, glo ELISA Kit, lyr ELISA Kit, pac ELISA Kit, wu:fb47h04 ELISA Kit, cadherin 2 ELISA Kit, cadherin 2 S homeolog ELISA Kit, cadherin 2, type 1, N-cadherin (neuronal) ELISA Kit, CDH2 ELISA Kit, cdh2.S ELISA Kit, cdh2 ELISA Kit, Cdh2 ELISA Kit

Hintergrund:

Protein Function: Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells, cadherins may thus contribute to the sorting of heterogeneous cell types. Acts as a regulator of neural stem cells quiescence by mediating anchorage of neural stem cells to ependymocytes in the adult subependymal zone: upon cleavage by MMP24, CDH2-mediated anchorage is affected, leading to modulate neural stem cell quiescence (PubMed:24952463). CDH2 may be involved in neuronal recognition mechanism. In hippocampal neurons, may regulate dendritic spine density. .

Background: Cadherin-2(CDH2), also known as neural cadherin(NCAD), is a protein that in humans is encoded by the CDH2 gene. It is a classical cadherin from the cadherin superfamily. This gene is mapped to 18q12.1. Cadherin-2 is expressed in the brain, skeletal and cardiac muscle. Cadherin-2 is commonly found in cancer cells and provides a mechanism for transendothelial migration. It is a calcium dependent cell-cell adhesion glycoprotein comprising five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. The protein functions during gastrulation and is required for establishment of left-right asymmetry. At certain central nervous system synapses, presynaptic to postsynaptic adhesion is mediated at least in part by this gene product.

Synonyms: Cadherin-2,Neural cadherin,N-cadherin,CD325,Cdh2,

Full Gene Name: Cadherin-2

Cellular Localisation: Cell membrane, Single-pass type I membrane protein.

Gen-ID: 12558

UniProt: P15116

Pathways: Regulation of Muscle Cell Differentiation, Cell-Cell Junction Organization, Synaptic Membrane