IL1RL1 ELISA Kit

Produktnummer ABIN1889354

Produktdetails IL1RL1 ELISA Kit

Target: IL1RL1 (Interleukin 1 Receptor-Like 1 (IL1RL1))

Bindungsspezifität: AA 27-332

Reaktivität: Maus

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 156-10.000 pg/mL

Untere Nachweisgrenze: 156 pg/mL

Applikation: ELISA

Verwendungszweck: Sandwich High Sensitivity ELISA kit for Quantitative Detection of Mouse IL1RL1/ST2

Marke: PicoKine™

Proben: Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)

Analytische Methode: Quantitative

Spezifität: Expression system for standard: NSO
Immunogen sequence: S27-R332

Kreuzreaktivität (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensitivität: <10pg/mL

Benötigtes Material: Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl

Immunogen: Expression system for standard: NSO
Immunogen sequence: S27-R332

Anwendungsinformationen

Applikationshinweise: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Kommentare:

Sequence similarities: Belongs to the interleukin-1 receptor family.

Tissue Specificity: Predominantly expressed in hematopoietic tissues, and in macrophage, erythroid, epithelial and fibroblast cell lines. .

Plattentyp: Pre-coated

Protokoll: mouse IL1RL1 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for IL1RL1 has been precoated onto 96-well plates. Standards(NSO, S27-R332) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for IL1RL1 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse IL1RL1 amount of sample captured in plate.

Testdurchführung:

Aliquot 0.1 mL per well of the 10,000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL, 156pg/mL mouse IL1RL1 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each mouse IL1RL1 standard solution and each sample be measured in duplicate.

Testpräzision:

• Sample 1: n=16, Mean(ng/ml): 0.57, Standard deviation: 0.029, CV(%): 5.1
• Sample 2: n=16, Mean(ng/ml): 2.33, Standard deviation: 0.086, CV(%): 3.7
• Sample 3: n=16, Mean(ng/ml): 4.25, Standard deviation: 0.136, CV(%): 3.2,
• Sample 1: n=24, Mean(ng/ml): 0.68, Standard deviation: 0.058, CV(%): 8.6
• Sample 2: n=24, Mean(ng/ml): 2.74, Standard deviation: 0.17, CV(%): 6.2
• Sample 3: n=24, Mean(ng/ml): 4.61, Standard deviation: 0.244, CV(%): 5.3

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: Avoid multiple freeze-thaw cycles.

Lagerung: -20 °C,4 °C

Informationen zur Lagerung: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles

Haltbarkeit: 12 months

Details zu IL1RL1

Target: IL1RL1 (Interleukin 1 Receptor-Like 1 (IL1RL1))

Andere Bezeichnung: IL1RL1 (IL1RL1 Produkte)

Synonyme: DER4 ELISA Kit, Fit-1 ELISA Kit, Ly84 ELISA Kit, ST2L ELISA Kit, St2 ELISA Kit, St2-rs1 ELISA Kit, T1 ELISA Kit, T1/ST2 ELISA Kit, FIT-1 ELISA Kit, IL33R ELISA Kit, ST2 ELISA Kit, ST2V ELISA Kit, FIT1 ELISA Kit, interleukin 1 receptor-like 1 ELISA Kit, interleukin 1 receptor like 1 ELISA Kit, Il1rl1 ELISA Kit, IL1RL1 ELISA Kit

Hintergrund:

Protein Function: Receptor for interleukin-33 (IL-33), its stimulation recruits MYD88, IRAK1, IRAK4, and TRAF6, followed by phosphorylation of MAPK3/ERK1 and/or MAPK1/ERK2, MAPK14, and MAPK8 (By similarity). Possibly involved in helper T-cell function. .

Background: Interleukin 1 receptor-like 1, also known as IL1RL1 or ST2, is a protein that in humans is encoded by the IL1RL1 gene. The protein encoded by this gene is a member of the interleukin 1 receptor family. Studies of the similar gene in mouse suggested that this receptor can be induced by proinflammatory stimuli, and may be involved in the function of helper T cells. TL1RL1 is necessary for endotoxin tolerance and, by inhibiting TLR responses, enhances Th2 responses. This gene, interleukin 1 receptor, type I(IL1R1), interleukin 1 receptor, type II(IL1R2) and interleukin 1 receptor-like 2(IL1RL2) form a cytokine receptor gene cluster in a region mapped to chromosome 2q12.

Synonyms: Interleukin-1 receptor-like 1,Lymphocyte antigen 84,Protein ST2,Protein T1,Il1rl1,Ly84, St2, Ste2,

Full Gene Name: Interleukin-1 receptor-like 1

Cellular Localisation: Cell membrane, Single-pass type I membrane protein.

Gen-ID: 17082

UniProt: P14719