APCS ELISA Kit
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- Target Alle APCS ELISA Kits anzeigen
- APCS (Amyloid P Component, Serum (APCS))
- Bindungsspezifität
- AA 21-224
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Reaktivität
- Maus
- Nachweismethode
- Colorimetric
- Methodentyp
- Sandwich ELISA
- Detektionsbereich
- 1.56-100 ng/mL
- Untere Nachweisgrenze
- 1.56 ng/mL
- Applikation
- ELISA
- Verwendungszweck
- Sandwich High Sensitivity ELISA kit for Quantitative Detection of Mouse SAP/PTX2
- Marke
- PicoKine™
- Proben
- Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)
- Analytische Methode
- Quantitative
- Spezifität
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Expression system for standard: NSO
Immunogen sequence: Q21-D224 - Kreuzreaktivität (Details)
- There is no detectable cross-reactivity with other relevant proteins.
- Sensitivität
- <50pg/mL
- Benötigtes Material
- Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
- Immunogen
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Expression system for standard: NSO
Immunogen sequence: Q21-D224 - Featured
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- Applikationshinweise
- Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
- Kommentare
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Sequence similarities: Belongs to the pentaxin family.
- Plattentyp
- Pre-coated
- Protokoll
- mouse SAP ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for SAP has been precoated onto 96-well plates. Standards(NSO, Q21-D224) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for SAP is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse SAP amount of sample captured in plate.
- Testdurchführung
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Aliquot 0.1 mL per well of the 100 ng/mL, 50 ng/mL, 25 ng/mL, 1.25 ng/mL, 6.25 ng/mL, 3.12 ng/mL, 1.56 ng/mL mouse SAP standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. We recommend that each mouse SAP standard solution and each sample is measured in duplicate.
- Testpräzision
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- Sample 1: n=16, Mean(ng/ml): 5.68, Standard deviation: 0.33, CV(%): 5.8
- Sample 2: n=16, Mean(ng/ml): 34, Standard deviation: 1.43, CV(%): 4.2
- Sample 3: n=16, Mean(ng/ml): 61, Standard deviation: 3.05, CV(%): 5,
- Sample 1: n=24, Mean(ng/ml): 5.2, Standard deviation: 0.39, CV(%): 7.5
- Sample 2: n=24, Mean(ng/ml): 39, Standard deviation: 2.11, CV(%): 5.4
- Sample 3: n=24, Mean(ng/ml): 74, Standard deviation: 4.59, CV(%): 6.2
- Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Handhabung
- Avoid multiple freeze-thaw cycles.
- Lagerung
- -20 °C,4 °C
- Informationen zur Lagerung
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
- Haltbarkeit
- 12 months
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- Target Alle APCS ELISA Kits anzeigen
- APCS (Amyloid P Component, Serum (APCS))
- Andere Bezeichnung
- APCS (APCS Produkte)
- Synonyme
- PTX2 ELISA Kit, SAP ELISA Kit, Sap ELISA Kit, APCS ELISA Kit, FP ELISA Kit, SAP(FP) ELISA Kit, apcs ELISA Kit, ptx2 ELISA Kit, sap ELISA Kit, amyloid P component, serum ELISA Kit, serum amyloid P-component ELISA Kit, amyloid P component, serum protein L homeolog ELISA Kit, APCS ELISA Kit, Apcs ELISA Kit, apcs.L ELISA Kit
- Hintergrund
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Background: Amyloid P component, serum(SAP), also known as PTX2 or APCS, is the identical serum form of amyloid P component(AP), a 25 kDa pentameric protein first identified as the pentagonal constituent of in vivo pathological deposits called "amyloid". It belongs to the pentraxins family, characterised by calcium dependent ligand binding and distinctive flattened beta-jellyroll structure similar to that of the legume lectins. This gene is mapped to 1q23.2. The binding of the encoded protein to proteins in the pathological amyloid cross-beta fold suggests its possible role as a chaperone. This protein is also thought to control the degradation of chromatin. It has been demonstrated that this protein binds to apoptotic cells at an early stage, which raises the possibility that it is involved in dealing with apoptotic cells in vivo.
Synonyms: Serum amyloid P-component,SAP,Apcs,Ptx2, Sap,
Full Gene Name: Serum amyloid P-component
Cellular Localisation: Secreted. - UniProt
- P12246
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