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CD13 ELISA Kit

ANPEP Reaktivität: Human AA 69-967 Colorimetric Sandwich ELISA 312-20.000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
Produktnummer ABIN1889381
  • Target Alle CD13 (ANPEP) ELISA Kits anzeigen
    CD13 (ANPEP) (Alanyl (Membrane) Aminopeptidase (ANPEP))
    Bindungsspezifität
    AA 69-967
    Reaktivität
    • 5
    • 4
    • 1
    • 1
    • 1
    • 1
    • 1
    Human
    Nachweismethode
    Colorimetric
    Methodentyp
    Sandwich ELISA
    Detektionsbereich
    312-20.000 pg/mL
    Untere Nachweisgrenze
    312 pg/mL
    Applikation
    ELISA
    Verwendungszweck
    Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human CD13/Aminopeptidase N
    Marke
    PicoKine™
    Proben
    Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)
    Analytische Methode
    Quantitative
    Spezifität
    Expression system for standard: NSO
    Immunogen sequence: K69-K967
    Kreuzreaktivität (Details)
    There is no detectable cross-reactivity with other relevant proteins.
    Sensitivität
    <10pg/mL
    Benötigtes Material
    Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
    Immunogen
    Expression system for standard: NSO
    Immunogen sequence: K69-K967
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  • Applikationshinweise
    Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
    Kommentare

    Tissue Specificity: Expressed in epithelial cells of the kidney, intestine, and respiratory tract, granulocytes, monocytes, fibroblasts, endothelial cells, cerebral pericytes at the blood- brain barrier, synaptic membranes of cells in the CNS. Also expressed in endometrial stromal cells, but not in the endometrial glandular cells. Found in the vasculature of tissues that undergo angiogenesis and in malignant gliomas and lymph node metastases from multiple tumor types but not in blood vessels of normal tissues. A soluble form has been found in plasma. It is found to be elevated in plasma and effusions of cancer patients.

    Plattentyp
    Pre-coated
    Protokoll
    human CD13 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for CD13 has been precoated onto 96-well plates. Standards(NSO, K69-K967) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CD13 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human CD13 amount of sample captured in plate.
    Testdurchführung

    Aliquot 0.1 mL per well of the 20,000pg/mL, 10,000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL human CD13 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human CD13 standard solution and each sample be measured in duplicate.

    Testpräzision
    • Sample 1: n=16, Mean(ng/ml): 2.98, Standard deviation: 0.158, CV(%): 5.3
    • Sample 2: n=16, Mean(ng/ml): 6.54, Standard deviation: 0.360, CV(%): 5.5
    • Sample 3: n=16, Mean(ng/ml): 12.73, Standard deviation: 0.751, CV(%): 5.9,
    • Sample 1: n=24, Mean(ng/ml): 3.27, Standard deviation: 0.190, CV(%): 5.8
    • Sample 2: n=24, Mean(ng/ml): 6.83, Standard deviation: 0.430, CV(%): 6.3
    • Sample 3: n=24, Mean(ng/ml): 13.76, Standard deviation: 0.922, CV(%): 6.7
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Handhabung
    Avoid multiple freeze-thaw cycles.
    Lagerung
    -20 °C,4 °C
    Informationen zur Lagerung
    Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
    Haltbarkeit
    12 months
  • Wang, Li, Sun, Ni, Huan, Huang, Li, Fan, Ren, Mao: "Triggering unfolded protein response by 2-Deoxy-D-glucose inhibits porcine epidemic diarrhea virus propagation." in: Antiviral research, Vol. 106, pp. 33-41, (2014) (PubMed).

  • Target Alle CD13 (ANPEP) ELISA Kits anzeigen
    CD13 (ANPEP) (Alanyl (Membrane) Aminopeptidase (ANPEP))
    Andere Bezeichnung
    ANPEP (ANPEP Produkte)
    Synonyme
    APN ELISA Kit, CD13 ELISA Kit, GP150 ELISA Kit, LAP1 ELISA Kit, P150 ELISA Kit, PEPN ELISA Kit, A2AP ELISA Kit, AAP ELISA Kit, ALPHA-2-PI ELISA Kit, API ELISA Kit, PLI ELISA Kit, AAT1 ELISA Kit, ALT1 ELISA Kit, GPT1 ELISA Kit, Gpt1 ELISA Kit, Apn ELISA Kit, pepN ELISA Kit, SC8E4A.13 ELISA Kit, 1300007J06Rik ELISA Kit, 2310022B03Rik ELISA Kit, ALT ELISA Kit, Gpt-1 ELISA Kit, AP-M ELISA Kit, AP-N ELISA Kit, Cd13 ELISA Kit, Apm ELISA Kit, KZP ELISA Kit, Lap1 ELISA Kit, rAPN ELISA Kit, APDE ELISA Kit, p150 ELISA Kit, anpep ELISA Kit, fb64c05 ELISA Kit, wu:fb64c05 ELISA Kit, zgc:136771 ELISA Kit, alanyl aminopeptidase, membrane ELISA Kit, serpin family F member 2 ELISA Kit, glutamic--pyruvic transaminase ELISA Kit, aminopeptidase N ELISA Kit, aminopeptidase ELISA Kit, aminopeptidase n ELISA Kit, glutamic pyruvic transaminase, soluble ELISA Kit, alanyl (membrane) aminopeptidase ELISA Kit, membrane alanyl aminopeptidase ELISA Kit, alanyl (membrane) aminopeptidase b ELISA Kit, alanyl aminopeptidase, membrane L homeolog ELISA Kit, ANPEP ELISA Kit, SERPINF2 ELISA Kit, GPT ELISA Kit, Gpt ELISA Kit, Apn2 ELISA Kit, Apn1 ELISA Kit, SCO2643 ELISA Kit, pepN ELISA Kit, Shewana3_0897 ELISA Kit, Alvin_1024 ELISA Kit, Ndas_3413 ELISA Kit, Saut_1544 ELISA Kit, Fbal_1877 ELISA Kit, ML5_3424 ELISA Kit, Despr_1988 ELISA Kit, Mesop_5365 ELISA Kit, Anpep ELISA Kit, LOC101744250 ELISA Kit, anpepb ELISA Kit, anpep.L ELISA Kit
    Hintergrund

    Protein Function: Broad specificity aminopeptidase. Plays a role in the final digestion of peptides generated from hydrolysis of proteins by gastric and pancreatic proteases. May play a critical role in the pathogenesis of cholesterol gallstone disease. May be involved in the metabolism of regulatory peptides of diverse cell types, responsible for the processing of peptide hormones, such as angiotensin III and IV, neuropeptides, and chemokines. Found to cleave antigen peptides bound to major histocompatibility complex class II molecules of presenting cells and to degrade neurotransmitters at synaptic junctions. Is also implicated as a regulator of IL-8 bioavailability in the endometrium, and therefore may contribute to the regulation of angiogenesis. Is used as a marker for acute myeloid leukemia and plays a role in tumor invasion. In case of human coronavirus 229E (HCoV-229E) infection, serves as receptor for HCoV-229E spike glycoprotein. Mediates as well human cytomegalovirus (HCMV) infection. .

    Background: Alanine aminopeptidase, also known as ANPEP or CD13. is an enzyme that is used as a biomarker to detect damage to the kidneys, and that may be used to help diagnose certain kidney disorders. It is mapped to 15q26.1. Aminopeptidase N is located in the small-intestinal and renal microvillar membrane, and also in other plasma membranes. In the small intestine, Aminopeptidase N plays a role in the final digestion of peptides generated from hydrolysis of proteins by gastric and pancreatic proteases, and it is also thought to be involved in the metabolism of regulatory peptides by diverse cell types, including small intestinal and renal tubular epithelial cells, macrophages, granulocytes, and synaptic membranes from the CNS. Petrovic et al showed that CD13 was required for endothelial cell invasion in response to bradykinin. Inhibition of CD13 abrogated internalization of bradykinin receptor B2 and reduced endothelial cell motility.

    Synonyms: Aminopeptidase N,AP-N,hAPN,3.4.11.2,Alanyl aminopeptidase,Aminopeptidase M,AP-M,Microsomal aminopeptidase,Myeloid plasma membrane glycoprotein CD13,gp150,CD13,ANPEP,APN, CD13, PEPN,

    Full Gene Name: Aminopeptidase N

    Cellular Localisation: Cell membrane, Single-pass type II membrane protein. Cytoplasm, cytosol . A soluble form has also been detected.
    Gen-ID
    290
    UniProt
    P15144
    Pathways
    Peptide Hormone Metabolism, Regulation of Systemic Arterial Blood Pressure by Hormones
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