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CCL9 ELISA Kit

Ccl9 Reaktivität: Maus AA 22-122 Colorimetric Sandwich ELISA 7.8-500 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
Produktnummer ABIN1889389
  • Target Alle CCL9 (Ccl9) ELISA Kits anzeigen
    CCL9 (Ccl9) (Chemokine (C-C Motif) Ligand 9 (Ccl9))
    Bindungsspezifität
    AA 22-122
    Reaktivität
    • 4
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Maus
    Nachweismethode
    Colorimetric
    Methodentyp
    Sandwich ELISA
    Detektionsbereich
    7.8-500 pg/mL
    Untere Nachweisgrenze
    7.8 pg/mL
    Applikation
    ELISA
    Verwendungszweck
    Sandwich High Sensitivity ELISA kit for Quantitative Detection of Mouse CCL9
    Marke
    PicoKine™
    Proben
    Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)
    Analytische Methode
    Quantitative
    Spezifität
    Expression system for standard: E.coli
    Immunogen sequence: Q22-Q122
    Kreuzreaktivität (Details)
    There is no detectable cross-reactivity with other relevant proteins.
    Sensitivität
    <1pg/mL
    Benötigtes Material
    Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
    Immunogen
    Expression system for standard: E.coli
    Immunogen sequence: Q22-Q122
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  • Applikationshinweise
    Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
    Kommentare

    Tissue Specificity: Expressed mainly in the liver, lung, and the thymus, although some expression has been detected in a wide variety of tissues except brain.

    Plattentyp
    Pre-coated
    Protokoll
    mouse CCL9 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for CCL9 has been precoated onto 96-well plates. Standards(E.coli, Q22-Q122) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CCL9 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse CCL9 amount of sample captured in plate.
    Testdurchführung

    Aliquot 0.1 mL per well of the 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.3pg/mL, 15.6pg/mL, 7.8pg/mL mouse CCL9 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. We recommend that each mouse CCL9 standard solution and each sample is measured in duplicate.

    Testpräzision
    • Sample 1: n=16, Mean(pg/ml): 34, Standard deviation: 1.19, CV(%): 3.5
    • Sample 2: n=16, Mean(pg/ml): 78, Standard deviation: 3.67, CV(%): 4.7
    • Sample 3: n=16, Mean(pg/ml): 294, Standard deviation: 14.99, CV(%): 5.1,
    • Sample 1: n=24, Mean(pg/ml): 35, Standard deviation: 1.68, CV(%): 4.8
    • Sample 2: n=24, Mean(pg/ml): 82, Standard deviation: 4.59, CV(%): 5.6
    • Sample 3: n=24, Mean(pg/ml): 302, Standard deviation: 19.03, CV(%): 6.3
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Handhabung
    Avoid multiple freeze-thaw cycles.
    Lagerung
    -20 °C,4 °C
    Informationen zur Lagerung
    Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
    Haltbarkeit
    12 months
  • Target Alle CCL9 (Ccl9) ELISA Kits anzeigen
    CCL9 (Ccl9) (Chemokine (C-C Motif) Ligand 9 (Ccl9))
    Andere Bezeichnung
    Ccl9 (Ccl9 Produkte)
    Synonyme
    CCF18 ELISA Kit, MRP-2 ELISA Kit, Scya10 ELISA Kit, Scya9 ELISA Kit, chemokine (C-C motif) ligand 9 ELISA Kit, Ccl9 ELISA Kit
    Hintergrund

    Protein Function: Monokine with inflammatory, pyrogenic and chemokinetic properties. Circulates at high concentrations in the blood of healthy animals. Binding to a high-affinity receptor activates calcium release in neutrophils. It also inhibits colony formation of bone marrow myeloid immature progenitors.

    Background: Chemokine(C-C motif) ligand 9(CCL9), also called MIP-1 gamma and MRP-2, is a small cytokine belonging to the CC chemokine family. CCL9 which found around Peyer's patches is secreted by follicle-associated epithelium(FAE), and it can attract dendritic cells that possess the cell surface molecule CD11b and the chemokine receptor CCR1. CCL9 is constitutively expressed in macrophages and myeloid cells. CCL9 can activate osteoclasts through its receptor CCR1(the most abundant chemokine receptor found on osteoclasts) suggesting an important role for CCL9 in bone resorption.

    Synonyms: C-C motif chemokine 9,CCF18,Macrophage inflammatory protein 1-gamma,MIP-1-gamma,Macrophage inflammatory protein-related protein 2,MRP-2,Small-inducible cytokine A9,CCL9(29-101),CCL9(30-101),CCL9(31-101),Ccl9,Mrp2, Scya10, Scya9,

    Full Gene Name: C-C motif chemokine 9

    Cellular Localisation: Secreted.
    Gen-ID
    20308
    UniProt
    P51670
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