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Relaxin 1 ELISA Kit

RLN1 Reaktivität: Maus AA 23-54 Colorimetric Sandwich ELISA 78-5000 pg/mL Cell Culture Supernatant, Plasma (heparin), Serum
Produktnummer ABIN1889393
  • Target Alle Relaxin 1 (RLN1) ELISA Kits anzeigen
    Relaxin 1 (RLN1)
    Bindungsspezifität
    AA 23-54, AA 161-185
    Reaktivität
    • 5
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    Maus
    Nachweismethode
    Colorimetric
    Methodentyp
    Sandwich ELISA
    Detektionsbereich
    78-5000 pg/mL
    Untere Nachweisgrenze
    78 pg/mL
    Applikation
    ELISA
    Verwendungszweck
    Sandwich High Sensitivity ELISA kit for Quantitative Detection of Mouse Relaxin 1
    Marke
    PicoKine™
    Proben
    Cell Culture Supernatant, Serum, Plasma (heparin)
    Analytische Methode
    Quantitative
    Spezifität
    Expression system for standard: E.coli
    Immunogen sequence: R23-R54(B)&E161-C185(A)
    Kreuzreaktivität (Details)
    There is no detectable cross-reactivity with other relevant proteins.
    Sensitivität
    <10pg/mL
    Benötigtes Material
    Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
    Immunogen
    Expression system for standard: E.coli
    Immunogen sequence: R23-R54(B)&E161-C185(A)
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  • Applikationshinweise
    Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
    Plattentyp
    Pre-coated
    Protokoll
    mouse Relaxin 1 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for Relaxin 1 has been precoated onto 96-well plates. Standards(E.coli, R23-R54(B) & E161-C185(A)) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Relaxin 1 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse Relaxin 1 amount of sample captured in plate.
    Testdurchführung

    Aliquot 0.1 mL per well of 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 313pg/mL, 156pg/mL, 78pg/mL mouse Relaxin 1 standard solutions into the pre-coated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum or plasma(heparin) to each empty well. See "Sample Dilution Guideline" above for details. We recommend that each mouse Relaxin 1 standard solution and each sample is measured in duplicate.

    Testpräzision
    • Sample 1: n=16, Mean(pg/ml): 688, Standard deviation: 45.41, CV(%): 6.6
    • Sample 2: n=16, Mean(pg/ml): 1402, Standard deviation: 60.3, CV(%): 4.3
    • Sample 3: n=16, Mean(pg/ml): 2366, Standard deviation: 130, CV(%): 5.5,
    • Sample 1: n=24, Mean(pg/ml): 651, Standard deviation: 48.2, CV(%): 7.4
    • Sample 2: n=24, Mean(pg/ml): 1583, Standard deviation: 95, CV(%): 6
    • Sample 3: n=24, Mean(pg/ml): 2425, Standard deviation: 174.6, CV(%): 7.2
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Handhabung
    Avoid multiple freeze-thaw cycles.
    Lagerung
    -20 °C,4 °C
    Informationen zur Lagerung
    Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
    Haltbarkeit
    12 months
  • Target Alle Relaxin 1 (RLN1) ELISA Kits anzeigen
    Relaxin 1 (RLN1)
    Andere Bezeichnung
    RLN1 (RLN1 Produkte)
    Synonyme
    H1 ELISA Kit, H1RLX ELISA Kit, RLXH1 ELISA Kit, bA12D24.3.1 ELISA Kit, bA12D24.3.2 ELISA Kit, Rln ELISA Kit, rlx ELISA Kit, RELAX ELISA Kit, RLX1 ELISA Kit, RNL1 ELISA Kit, RLN1 ELISA Kit, RLX ELISA Kit, relaxin 1 ELISA Kit, RLN1 ELISA Kit, Rln1 ELISA Kit
    Hintergrund

    Protein Function: Relaxin is an ovarian hormone that acts with estrogen to produce dilatation of the birth canal in many mammals.

    Background: Relaxin 1 is a member of relaxin-like peptide family. Relaxin gene maps to mouse chromosome 19 near D19Mit23. Relaxin is a peptide hormone produced by the corpora lutea of ovaries during pregnancy in many mammalian species, including man. Relaxin widens the pubic bone and facilitates labor, it also softens the cervix(cervical ripening), and relaxes the uterine musculature. However, its significance may reach much further. Relaxin affects collagen metabolism, inhibiting collagen synthesis and enhancing its breakdown by increasing matrix metalloproteinases. It also enhances angiogenesis and is a potent renal vasodilator.

    Synonyms: Prorelaxin 1,Relaxin B chain,Relaxin A chain,Rln1,Rln, Rlx,

    Full Gene Name: Prorelaxin 1

    Cellular Localisation: Secreted.
    Gen-ID
    19773
    UniProt
    P47932
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