Relaxin 1 ELISA Kit

Produktnummer ABIN1889393

Produktdetails Relaxin 1 ELISA Kit

Target: Relaxin 1 (RLN1)

Bindungsspezifität: AA 23-54, AA 161-185

Reaktivität: Maus

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 78-5000 pg/mL

Untere Nachweisgrenze: 78 pg/mL

Applikation: ELISA

Verwendungszweck: Sandwich High Sensitivity ELISA kit for Quantitative Detection of Mouse Relaxin 1

Marke: PicoKine™

Proben: Cell Culture Supernatant, Serum, Plasma (heparin)

Analytische Methode: Quantitative

Spezifität: Expression system for standard: E.coli
Immunogen sequence: R23-R54(B)&E161-C185(A)

Kreuzreaktivität (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensitivität: <10pg/mL

Benötigtes Material: Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl

Immunogen: Expression system for standard: E.coli
Immunogen sequence: R23-R54(B)&E161-C185(A)

Anwendungsinformationen

Applikationshinweise: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Plattentyp: Pre-coated

Protokoll: mouse Relaxin 1 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for Relaxin 1 has been precoated onto 96-well plates. Standards(E.coli, R23-R54(B) & E161-C185(A)) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Relaxin 1 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse Relaxin 1 amount of sample captured in plate.

Testdurchführung:

Aliquot 0.1 mL per well of 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 313pg/mL, 156pg/mL, 78pg/mL mouse Relaxin 1 standard solutions into the pre-coated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum or plasma(heparin) to each empty well. See "Sample Dilution Guideline" above for details. We recommend that each mouse Relaxin 1 standard solution and each sample is measured in duplicate.

Testpräzision:

• Sample 1: n=16, Mean(pg/ml): 688, Standard deviation: 45.41, CV(%): 6.6
• Sample 2: n=16, Mean(pg/ml): 1402, Standard deviation: 60.3, CV(%): 4.3
• Sample 3: n=16, Mean(pg/ml): 2366, Standard deviation: 130, CV(%): 5.5,
• Sample 1: n=24, Mean(pg/ml): 651, Standard deviation: 48.2, CV(%): 7.4
• Sample 2: n=24, Mean(pg/ml): 1583, Standard deviation: 95, CV(%): 6
• Sample 3: n=24, Mean(pg/ml): 2425, Standard deviation: 174.6, CV(%): 7.2

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: Avoid multiple freeze-thaw cycles.

Lagerung: -20 °C,4 °C

Informationen zur Lagerung: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles

Haltbarkeit: 12 months

Details zu Relaxin 1

Target: Relaxin 1 (RLN1)

Andere Bezeichnung: RLN1 (RLN1 Produkte)

Synonyme: H1 ELISA Kit, H1RLX ELISA Kit, RLXH1 ELISA Kit, bA12D24.3.1 ELISA Kit, bA12D24.3.2 ELISA Kit, Rln ELISA Kit, rlx ELISA Kit, RELAX ELISA Kit, RLX1 ELISA Kit, RNL1 ELISA Kit, RLN1 ELISA Kit, RLX ELISA Kit, relaxin 1 ELISA Kit, RLN1 ELISA Kit, Rln1 ELISA Kit

Hintergrund:

Protein Function: Relaxin is an ovarian hormone that acts with estrogen to produce dilatation of the birth canal in many mammals.

Background: Relaxin 1 is a member of relaxin-like peptide family. Relaxin gene maps to mouse chromosome 19 near D19Mit23. Relaxin is a peptide hormone produced by the corpora lutea of ovaries during pregnancy in many mammalian species, including man. Relaxin widens the pubic bone and facilitates labor, it also softens the cervix(cervical ripening), and relaxes the uterine musculature. However, its significance may reach much further. Relaxin affects collagen metabolism, inhibiting collagen synthesis and enhancing its breakdown by increasing matrix metalloproteinases. It also enhances angiogenesis and is a potent renal vasodilator.

Synonyms: Prorelaxin 1,Relaxin B chain,Relaxin A chain,Rln1,Rln, Rlx,

Full Gene Name: Prorelaxin 1

Cellular Localisation: Secreted.

Gen-ID: 19773

UniProt: P47932