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FOLR1 ELISA Kit

FOLR1 Reaktivität: Maus AA 25-232 Colorimetric Sandwich ELISA 46.9-3000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
Produktnummer ABIN1889400
  • Target Alle FOLR1 ELISA Kits anzeigen
    FOLR1 (Folate Receptor 1 (Adult) (FOLR1))
    Bindungsspezifität
    AA 25-232
    Reaktivität
    • 6
    • 4
    • 1
    • 1
    Maus
    Nachweismethode
    Colorimetric
    Methodentyp
    Sandwich ELISA
    Detektionsbereich
    46.9-3000 pg/mL
    Untere Nachweisgrenze
    46.9 pg/mL
    Applikation
    ELISA
    Verwendungszweck
    Sandwich High Sensitivity ELISA kit for Quantitative Detection of Mouse FOLR1
    Marke
    PicoKine™
    Proben
    Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)
    Analytische Methode
    Quantitative
    Spezifität
    Expression system for standard: E.coli
    Immunogen sequence: T25-S232
    Kreuzreaktivität (Details)
    There is no detectable cross-reactivity with other relevant proteins.
    Sensitivität
    <10pg/mL
    Benötigtes Material
    Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
    Immunogen
    Expression system for standard: E.coli
    Immunogen sequence: T25-S232
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  • Applikationshinweise
    Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
    Kommentare

    Tissue Specificity: Detected in kidney proximal tubules (at protein level). .

    Plattentyp
    Pre-coated
    Protokoll
    mouse FOLR1 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for FOLR1 has been precoated onto 96-well plates. Standards(E.coli, T25-S232)and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for FOLR1 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse FOLR1 amount of sample captured in plate.
    Testdurchführung

    Aliquot 0.1 mL per well of the 3000pg/mL, 1500pg/mL, 750pg/mL,375.pg/mL, 187.5pg/mL, 93.8pg/mL, 46.9pg/mL mouse FOLR1 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernatants, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. We recommend that each mouse FOLR1 standard solution and each sample is measured in duplicate.

    Testpräzision
    • Sample 1: n=16, Mean(pg/ml): 378, Standard deviation: 17.01, CV(%): 4.5
    • Sample 2: n=16, Mean(pg/ml): 1024, Standard deviation: 32.77, CV(%): 3.2
    • Sample 3: n=16, Mean(pg/ml): 1915, Standard deviation: 88.09, CV(%): 4.6,
    • Sample 1: n=24, Mean(pg/ml): 379, Standard deviation: 20.47, CV(%): 5.4
    • Sample 2: n=24, Mean(pg/ml): 1025, Standard deviation: 66.63, CV(%): 6.5
    • Sample 3: n=24, Mean(pg/ml): 1978, Standard deviation: 112.8, CV(%): 5.7
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Handhabung
    Avoid multiple freeze-thaw cycles.
    Lagerung
    -20 °C,4 °C
    Informationen zur Lagerung
    Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
    Haltbarkeit
    12 months
  • Target Alle FOLR1 ELISA Kits anzeigen
    FOLR1 (Folate Receptor 1 (Adult) (FOLR1))
    Andere Bezeichnung
    FOLR1 (FOLR1 Produkte)
    Synonyme
    FBP ELISA Kit, FOLR ELISA Kit, fbp ELISA Kit, folr ELISA Kit, folr1 ELISA Kit, FBP1 ELISA Kit, Folbp-1 ELISA Kit, Folbp1 ELISA Kit, Fbp1 ELISA Kit, FOLR1 ELISA Kit, Folr1 ELISA Kit, folate receptor 1 ELISA Kit, folate receptor 1 (adult) ELISA Kit, folate receptor 1 (adult) L homeolog ELISA Kit, zgc:165502 ELISA Kit, Folate receptor alpha ELISA Kit, folate receptor alpha ELISA Kit, FOLR1 ELISA Kit, folr1 ELISA Kit, folr1.L ELISA Kit, zgc:165502 ELISA Kit, Folr1 ELISA Kit, LOC100066084 ELISA Kit, LOC100725439 ELISA Kit
    Hintergrund

    Protein Function: Binds to folate and reduced folic acid derivatives and mediates delivery of 5-methyltetrahydrofolate and folate analogs into the interior of cells. Has high affinity for folate and folic acid analogs at neutral pH . Exposure to slightly acidic pH after receptor endocytosis triggers a conformation change that strongly reduces its affinity for folates and mediates their release. Required for normal embryonic development and normal cell proliferation. Required for renal folate reabsorption. .

    Background: Folate receptor 1 adult is a protein that in humans is encoded by the FOLR1 gene. The protein encoded by this gene is a member of the folate receptor(FOLR) family. It is mapped to 11q13.4. FOLR1 expression in Jurkat cells facilitated MBG or EBO entry, and FR-blocking reagents inhibited infection of MBG or EBO. This gene product is a secreted protein that either anchors to membranes via a glycosyl-phosphatidylinositol linkage or exists in a soluble form. The FOLR1 gene encodes the adult folate receptor, or folate-binding protein(FBP), which has a high affinity for folic acid and for several reduced folic acid derivatives, and mediates delivery of 5-methyltetrahydrofolate to the interior of cells. FOLR1 is also an important regulator of milk protein synthesis.

    Synonyms: Folate receptor alpha,FR-alpha,Folate receptor 1,Folate-binding protein 1,Folr1,Fbp1, Folbp1,

    Full Gene Name: Folate receptor alpha

    Cellular Localisation: Cell membrane, Lipid-anchor, GPI-anchor. Secreted . Cytoplasmic vesicle . Cytoplasmic vesicle, clathrin-coated vesicle . Endosome . Apical cell membrane. Endocytosed into cytoplasmic vesicles and then recycled to the cell membrane (By similarity). Detected at proximal tubule apical membranes..
    Gen-ID
    14275
    UniProt
    P35846
    Pathways
    Dicarboxylic Acid Transport
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