LUM ELISA Kit

Produktnummer ABIN1889401

Produktdetails LUM ELISA Kit

Target: LUM (Lumican (LUM))

Bindungsspezifität: AA 19-338

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 156-10.000 pg/mL

Untere Nachweisgrenze: 156 pg/mL

Applikation: ELISA

Verwendungszweck: Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human Lumican

Marke: PicoKine™

Proben: Cell Culture Supernatant, Serum, Plasma (heparin)

Analytische Methode: Quantitative

Spezifität: Expression system for standard: NSO
Immunogen sequence: Q19-N338

Kreuzreaktivität (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensitivität: <10pg/mL

Benötigtes Material: Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl

Immunogen: Expression system for standard: NSO
Immunogen sequence: Q19-N338

Anwendungsinformationen

Applikationshinweise: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Kommentare:

Sequence similarities: Belongs to the small leucine-rich proteoglycan (SLRP) family. SLRP class II subfamily.

Tissue Specificity: Cornea and other tissues.

Plattentyp: Pre-coated

Protokoll: human Lumican ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for Lumican has been precoated onto 96-well plates. Standards(NSO, Q19-N338) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Lumican is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human Lumican amount of sample captured in plate.

Testdurchführung:

Aliquot 0.1 mL per well of the 10,000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL, 156pg/mL human Lumican standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human Lumican standard solution and each sample be measured in duplicate.

Testpräzision:

• Sample 1: n=16, Mean(pg/ml): 1433, Standard deviation: 80.25, CV(%): 5.6
• Sample 2: n=16, Mean(pg/ml): 4327, Standard deviation: 268.3, CV(%): 6.2
• Sample 3: n=16, Mean(pg/ml): 6957, Standard deviation: 313.1, CV(%): 4.5,
• Sample 1: n=24, Mean(pg/ml): 1724, Standard deviation: 115.5, CV(%): 6.7
• Sample 2: n=24, Mean(pg/ml): 4683, Standard deviation: 332.5, CV(%): 7.1
• Sample 3: n=24, Mean(pg/ml): 7234, Standard deviation: 419.6, CV(%): 5.8

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: Avoid multiple freeze-thaw cycles.

Lagerung: -20 °C,4 °C

Informationen zur Lagerung: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles

Haltbarkeit: 12 months

Details zu LUM

Target: LUM (Lumican (LUM))

Andere Bezeichnung: LUM (LUM Produkte)

Synonyme: MGC64527 ELISA Kit, zgc:86661 ELISA Kit, ldc ELISA Kit, slrr2d ELISA Kit, DKFZp468L1116 ELISA Kit, LDC ELISA Kit, SLRR2D ELISA Kit, Ldc ELISA Kit, lumican ELISA Kit, lumican L homeolog ELISA Kit, LUM ELISA Kit, lum.L ELISA Kit, lum ELISA Kit, CpipJ_CPIJ010209 ELISA Kit, Lum ELISA Kit

Hintergrund:

Background: Lumican, also known as LUM, is a protein which in humans is encoded by the LUM gene. This gene is a member of the small interstitial proteoglycan gene(SIPG) family, and it is a keratan sulfate proteoglycan which presents large quantities in the corneal stroma and in interstitial collagenous matrices of the heart, aorta, skeletal muscle, skin, and intervertebral discs. Lumican is mapped to 12q21.33, it interacts with collagen and limits growth of fibrils in diameter. In the cornea, Lumican not only interacts with collagen molecules to limit fibril growth, but also plays a critical role in the regular spacing of fibrils and acquisition of corneal transparency by virtue of its keratan sulfate-containing glycosaminoglycan side chains LDC.

Synonyms: Lumican,Keratan sulfate proteoglycan lumican,KSPG lumican,LUM,LDC, SLRR2D,

Full Gene Name: Lumican

Cellular Localisation: Secreted, extracellular space, extracellular matrix.

Gen-ID: 4060

UniProt: P51884

Pathways: Glycosaminoglycan Metabolic Process