TNFRSF11A ELISA Kit
-
- Target Alle TNFRSF11A ELISA Kits anzeigen
- TNFRSF11A (Tumor Necrosis Factor Receptor Superfamily, Member 11a, NFKB Activator (TNFRSF11A))
- Bindungsspezifität
- AA 30-213
-
Reaktivität
- Ratte
- Nachweismethode
- Colorimetric
- Methodentyp
- Sandwich ELISA
- Detektionsbereich
- 62.5-4000 pg/mL
- Untere Nachweisgrenze
- 62.5 pg/mL
- Applikation
- ELISA
- Verwendungszweck
- Sandwich High Sensitivity ELISA kit for Quantitative Detection of Rat RANK
- Marke
- PicoKine™
- Proben
- Cell Culture Supernatant, Serum
- Analytische Methode
- Quantitative
- Spezifität
-
Expression system for standard: NSO
Immunogen sequence: Q30-P213 - Kreuzreaktivität (Details)
- There is no detectable cross-reactivity with other relevant proteins.
- Sensitivität
- <10pg/mL
- Benötigtes Material
- Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
- Immunogen
-
Expression system for standard: NSO
Immunogen sequence: Q30-P213 - Featured
- Zu unserem meistverkauften TNFRSF11A ELISA Kit
- Top Product
- Discover our top product TNFRSF11A ELISA Kit
-
-
- Applikationshinweise
- Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
- Plattentyp
- Pre-coated
- Protokoll
- rat RANK ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for RANK has been precoated onto 96-well plates. Standards(NSO, Q30-P213) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for RANK is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the rat RANK amount of sample captured in plate.
- Testdurchführung
-
Aliquot 0.1 mL per well of the 4000pg/mL, 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL rat RANK standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of rat cell culture supernates or serum to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each rat RANK standard solution and each sample be measured in duplicate.
- Testpräzision
-
- Sample 1: n=16, Mean(pg/ml): 880, Standard deviation: 36.96, CV(%): 4.2
- Sample 2: n=16, Mean(pg/ml): 1954, Standard deviation: 107.5, CV(%): 5.5
- Sample 3: n=16, Mean(pg/ml): 2755, Standard deviation: 159.8, CV(%): 5.8,
- Sample 1: n=24, Mean(pg/ml): 950, Standard deviation: 51.3, CV(%): 5.4
- Sample 2: n=24, Mean(pg/ml): 2160, Standard deviation: 138.2, CV(%): 6.4
- Sample 3: n=24, Mean(pg/ml): 3052, Standard deviation: 228.9, CV(%): 7.5
- Beschränkungen
- Nur für Forschungszwecke einsetzbar
-
- Handhabung
- Avoid multiple freeze-thaw cycles.
- Lagerung
- -20 °C,4 °C
- Informationen zur Lagerung
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
- Haltbarkeit
- 12 months
-
- Target Alle TNFRSF11A ELISA Kits anzeigen
- TNFRSF11A (Tumor Necrosis Factor Receptor Superfamily, Member 11a, NFKB Activator (TNFRSF11A))
- Andere Bezeichnung
- TNFRSF11A (TNFRSF11A Produkte)
- Synonyme
- RANK ELISA Kit, RGD1563614 ELISA Kit, TNFRSF11A ELISA Kit, Ly109 ELISA Kit, ODFR ELISA Kit, OFE ELISA Kit, Rank ELISA Kit, TRANCE-R ELISA Kit, mRANK ELISA Kit, CD265 ELISA Kit, FEO ELISA Kit, LOH18CR1 ELISA Kit, OPTB7 ELISA Kit, OSTS ELISA Kit, PDB2 ELISA Kit, TRANCER ELISA Kit, TNF receptor superfamily member 11A ELISA Kit, TNF receptor superfamily member 11a ELISA Kit, tumor necrosis factor receptor superfamily, member 11a, NFKB activator ELISA Kit, Tnfrsf11a ELISA Kit, TNFRSF11A ELISA Kit
- Hintergrund
-
Background: Receptor Activator of Nuclear Factor i° B (RANK), also known as TRANCE Receptor, is a type I membrane protein that is expressed on the surface of osteoclasts and is involved in their activation upon ligand binding. RANK is a recently described TNF receptor family member, and its ligand, RANKL, promote survival of dendritic cells and differentiation of osteoclasts. RANK contains 383 amino acids in its intracellular domain(residues 234-616), which contain three putative TRAF-binding domains(termed I, II, and III). RANK interacts with various TRAFs through distinct motifs and activates NF-kappaB via a novel TRAF6 interaction motif, which then activates NIK, thus leading to NF-kappaB activation, whereas RANK most likely activates JNK through a TRAF2-interacting region in RANK. The standard in this kit is recombinant human RANK with the sequence of Q29-G213 aa. It is a dipolymer which compose of two chains, and the molecular weight of each is 48kda.
Synonyms: Protein Tnfrsf11a ,Tnfrsf11a ,
Full Gene Name: tumor necrosis factor receptor superfamily, member 11a, NFKB activator
- Gen-ID
- 498206
- UniProt
- F1M8Z6
- Pathways
- NF-kappaB Signalweg
-