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CD48 ELISA Kit

CD48 Reaktivität: Maus AA 23-217 Colorimetric Sandwich ELISA 62.5-4000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
Produktnummer ABIN1889451
  • Target Alle CD48 ELISA Kits anzeigen
    CD48
    Bindungsspezifität
    AA 23-217
    Reaktivität
    • 5
    • 4
    • 2
    Maus
    Nachweismethode
    Colorimetric
    Methodentyp
    Sandwich ELISA
    Detektionsbereich
    62.5-4000 pg/mL
    Untere Nachweisgrenze
    62.5 pg/mL
    Applikation
    ELISA
    Verwendungszweck
    Sandwich High Sensitivity ELISA kit for Quantitative Detection of Mouse CD48
    Marke
    PicoKine™
    Proben
    Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)
    Analytische Methode
    Quantitative
    Spezifität
    Expression system for standard: NSO
    Immunogen sequence: F23-S217
    Kreuzreaktivität (Details)
    There is no detectable cross-reactivity with other relevant proteins.
    Sensitivität
    <10pg/mL
    Benötigtes Material
    Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
    Immunogen
    Expression system for standard: NSO
    Immunogen sequence: F23-S217
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  • Applikationshinweise
    Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
    Kommentare

    Sequence similarities: Contains 2 Ig-like C2-type (immunoglobulin-like) domains.

    Plattentyp
    Pre-coated
    Protokoll
    mouse CD48 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for CD48 has been precoated onto 96-well plates. Standards(NSO, F23-S217) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CD48 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse CD48 amount of sample captured in plate.
    Testdurchführung

    Aliquot 0.1 mL per well of the 4000pg/mL, 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL mouse CD48 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each mouse CD48 standard solution and each sample be measured in duplicate.

    Testpräzision
    • Sample 1: n=16, Mean(pg/ml): 382, Standard deviation: 13.37, CV(%): 3.5
    • Sample 2: n=16, Mean(pg/ml): 1554, Standard deviation: 65.27, CV(%): 4.2
    • Sample 3: n=16, Mean(pg/ml): 2790, Standard deviation: 131.13, CV(%): 4.7,
    • Sample 1: n=24, Mean(pg/ml): 426, Standard deviation: 19.17, CV(%): 4.5
    • Sample 2: n=24, Mean(pg/ml): 1713, Standard deviation: 90.79, CV(%): 5.3
    • Sample 3: n=24, Mean(pg/ml): 3225, Standard deviation: 187.05, CV(%): 5.8
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Handhabung
    Avoid multiple freeze-thaw cycles.
    Lagerung
    -20 °C,4 °C
    Informationen zur Lagerung
    Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
    Haltbarkeit
    12 months
  • Target Alle CD48 ELISA Kits anzeigen
    CD48
    Andere Bezeichnung
    CD48 (CD48 Produkte)
    Synonyme
    AI449234 ELISA Kit, AW610730 ELISA Kit, BCM1 ELISA Kit, BLAST ELISA Kit, BLAST-1 ELISA Kit, BLAST1 ELISA Kit, Bcm-1 ELISA Kit, MEM-102 ELISA Kit, SLAMF2 ELISA Kit, Sgp-60 ELISA Kit, hCD48 ELISA Kit, mCD48 ELISA Kit, CD48 antigen ELISA Kit, Cd48 molecule ELISA Kit, CD48 molecule ELISA Kit, Cd48 ELISA Kit, CD48 ELISA Kit
    Hintergrund

    Protein Function: Ligand for CD2. Might facilitate interaction between activated lymphocytes. Probably involved in regulating T-cell activation.

    Background: CD48 antigen(Cluster of Differentiation 48), also known as BLAST-1 or SLAMF2, is a protein that in humans is encoded by the CD48 gene. CD48 is a member of the CD2 subfamily of the immunoglobulin superfamily(IgSF) which includes SLAM(signaling lymphocyte activation molecules) proteins, such as CD84, CD150, CD229 and CD244. It is mapped to 1q23.3. CD48 is found on the surface of lymphocytes and other immune cells, dendritic cells and endothelial cells, and participates in activation and differentiation pathways in these cells. The encoded protein does not have a transmembrane domain, however, but is held at the cell surface by a GPI anchor via a C-terminal domain which maybe cleaved to yield a soluble form of the receptor.

    Synonyms: CD48 antigen,BCM1 surface antigen,BLAST-1,HM48-1,MRC OX-45 surface antigen,SLAM family member 2,SLAMF2,Signaling lymphocytic activation molecule 2,sgp-60,CD48,Cd48,Bcm-1,

    Full Gene Name: CD48 antigen

    Cellular Localisation: Cell membrane, Lipid-anchor, GPI-anchor.
    Gen-ID
    12506
    UniProt
    P18181
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