ALOX12 ELISA Kit
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- Target Alle ALOX12 ELISA Kits anzeigen
- ALOX12 (Arachidonate 12-Lipoxygenase (ALOX12))
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Reaktivität
- Human
- Nachweismethode
- Colorimetric
- Methodentyp
- Sandwich ELISA
- Detektionsbereich
- 0.31-20 ng/mL
- Untere Nachweisgrenze
- 0.31 ng/mL
- Applikation
- ELISA
- Marke
- BioAssay™
- Sensitivität
- < 0.116 ng/mL
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- Plattentyp
- Pre-coated
- Protokoll
- Arachidonate-12-Lipoxygenase (ALOX12) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Arachidonate-12-Lipoxygenase (ALOX12). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Arachidonate-12-Lipoxygenase (ALOX12). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Arachidonate-12-Lipoxygenase (ALOX12), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Arachidonate-12-Lipoxygenase (ALOX12) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
- Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Lagerung
- 4 °C/-20 °C
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- Target Alle ALOX12 ELISA Kits anzeigen
- ALOX12 (Arachidonate 12-Lipoxygenase (ALOX12))
- Andere Bezeichnung
- Arachidonate-12-Lipoxygenase (ALOX12) (ALOX12 Produkte)
- Pathways
- Positive Regulation of Endopeptidase Activity
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