CD235a/GYPA ELISA Kit

Produktnummer ABIN2043581

Produktdetails CD235a/GYPA ELISA Kit

Target: CD235a/GYPA (GYPA) (Glycophorin A (GYPA))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.31-20 ng/mL

Untere Nachweisgrenze: 0.31 ng/mL

Applikation: ELISA

Marke: BioAssay™

Sensitivität: < 0.134 ng/mL

Anwendungsinformationen

Plattentyp: Pre-coated

Protokoll: Glycophorin A (GYPA) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Glycophorin A (GYPA). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Glycophorin A (GYPA). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Glycophorin A (GYPA), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Glycophorin A (GYPA) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C/-20 °C

Details zu CD235a/GYPA

Target: CD235a/GYPA (GYPA) (Glycophorin A (GYPA))

Abstract: GYPA Produkte

Pathways: Maintenance of Protein Location