Rhodopsin ELISA Kit

Produktnummer ABIN2044439

Produktdetails Rhodopsin ELISA Kit

Target: Rhodopsin (RHO)

Reaktivität: Chemical

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 1.56-100 ng/mL

Untere Nachweisgrenze: 1.56 ng/mL

Applikation: ELISA

Marke: BioAssay™

Sensitivität: < 0.64 ng/mL

Anwendungsinformationen

Plattentyp: Pre-coated

Protokoll: Rhodopsin (RHO) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rhodopsin (RHO). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Rhodopsin (RHO). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rhodopsin (RHO), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Rhodopsin (RHO) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C/-20 °C

Details zu Rhodopsin

Target: Rhodopsin (RHO)

Abstract: RHO Produkte

Substanzklasse: Chemical

Pathways: WNT Signalweg, Sensory Perception of Sound, Regulation of G-Protein Coupled Receptor Protein Signaling, Phototransduction