ADIPOQ ELISA Kit

Produktnummer ABIN2472064

Human ADIPOQ ELISA Kit, Colorimetric Assay für die Quantifizierung von Human ADIPOQ.

Kurzübersicht für ADIPOQ ELISA Kit (ABIN2472064)

Target: ADIPOQ (Adiponectin (ADIPOQ))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.44 pg/mL - 4000 pg/mL

Applikation: ELISA

Proben: Serum, Plasma, Cell Culture Supernatant

Produktdetails ADIPOQ ELISA Kit

Untere Nachweisgrenze: 0.44 pg/mL

Verwendungszweck: This assay employs the quantitative sandwich enzyme immunoassay technique for quantitative detection.

Analytische Methode: Quantitative

Spezifität: Human Adiponectin ELISA Kit

Bestandteile:

• Plate
• Standard
• Diluent

Benötigtes Material: Microplate reader capable of measuring absorbance at 450 nm, with correction wavelength set at 570 nm or 630 nm.
Pipettes and pipette tips.
50 μL to 300 μL adjustable multichannel micropipette with disposable tips.
Multichannel micropipette reservoir.
Beakers, flasks, cylinders necessary for preparation of reagents.
Deionized or distilled water.
Polypropylene test tubes for dilution.

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Probenmenge: 100 μL

Testdauer: 3 - 4 h

Plattentyp: Pre-coated

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: protect your eyes

Lagerung: 4 °C

Informationen zur Lagerung: Store kit reagents between 2 and 8 °C. Immediately after use remaining reagents should be returned to cold storage (2 to 8 °C).
Expiry of the kit and reagents is stated on labels. Expiration date of the kit components can only be guaranteed if the components are stored properly, and if, in case of repeated use of one component, this reagent is not contaminated by the first handling.
Unopened Kit: Store at 2 - 8 °C (See expiration date on the label).
Opened/Reconstituted Reagents: Up to 1 month at 2 - 8 °C.
Reconstituted Standard: Up to 1 month at ≤ -20 °C in a manual defrost freezer. Avoid repeated freeze-thaw cycles.
Microplate Wells: Up to 1 month at 2 - 8 °C. Return unused strips to the foil pouch containing the desiccant pack, reseal along entire edge to maintain plate integrity. Provided this is within the expiration date of the kit

Details zu ADIPOQ

Target: ADIPOQ (Adiponectin (ADIPOQ))

Andere Bezeichnung: Adiponectin

Hintergrund: Adiponectin, also known as gelatin-binding protein of 28 kDa (GBP-28), adipose most abundant gene transcript 1 (apM1), Adipocyte complement-related protein of 30 kDa (Acrp30), and AdipoQ, is a protein involved in regulating glucose levels as well as fatty acid breakdown. There are four distinct regions of adiponectin: a short signal sequence that targets the hormone for secretion outside the cell, a short region that varies between species, a 65-amino acid region with similarity to collagenous proteins and a globular domain.

NCBI Accession: NM_001177800

UniProt: Q15848