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Histamine ELISA Kit

HIS Reaktivität: Human Colorimetric Cell Culture Cells, Plasma, Urine

Produktnummer ABIN2648672

Datenblatt (PDF)

Target Alle Histamine (HIS) ELISA Kits anzeigen

Histamine (HIS)
Reaktivität
6

3

2

1

1

1

1

1

1

1

1

1

1

1
Human

Nachweismethode

Colorimetric

Applikation

ELISA

Proben

Plasma, Urine, Cell Culture Cells

Sensitivität

0.02 ng/mL

Produktmerkmale

Histamine plays a major role in the initial phase of an anaphylactic reaction. The quantification of histamine in plasma after allergen administration is of clinical interest. Histamine is part of the immune response to foreign pathogens and it increases the permeability of the capillaries to white blood cells and other proteins in order to allow them to engage foreign invaders in the affected tissues. Responsible for the biological effects of histamine in tissue are the activation of different surface receptors, for instance H1, H2 and H3. Histamine is involved in the regulating physiological function in the gut and acting as a neurotransmitter.

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Applikationshinweise

Optimal working dilution should be determined by the investigator.

Probenmenge

50 μL

Testdauer

2.5 h

Plattentyp

Pre-coated

Protokoll

The competitive Histamine ELISA kit uses the microtiter plate format. Histamine is bound to the solid phase of the microtiter plate. Acylated histamine and solid phase bound histamine compete for a fixed number of antiserum binding sites. When the system is in equilibrium, free antigen and free antigen-antiserum complexes are removed by washing. The antibody bound to the solid phase histamine is detected by anti-rabbit/peroxidase. The substrate TMB / peroxidase reaction is monitored at 450 nm. The amount of antibody bound to the solid phase histamine is inversely proportional to the histamine concentration of the sample.

Beschränkungen

Nur für Forschungszwecke einsetzbar
Lagerung

4 °C
Target Alle Histamine (HIS) ELISA Kits anzeigen

Histamine (HIS)

Andere Bezeichnung

Histamine (HIS Produkte)

Substanzklasse

Chemical