APC Annexin V Apoptosis Detection Kit with PI

Produktnummer ABIN2669211

Produktdetails

Target: Annexin V (ANXA5) (Annexin A5 (ANXA5))

Reaktivität: Säugetier

Konjugat: APC

Applikation: Multiplex Assay (MA)

Produktmerkmale: APC Annexin V Apoptosis Detection Kit with PI has been specifically designed for the identification of apoptotic and necrotic cells. Annexin V (or Annexin A5) is a member of the annexin family of intracellular proteins that binds to phosphatidylserine (PS) in a calcium-dependent manner. PS is normally only found on the intracellular leaflet of the plasma membrane in healthy cells, but during early apoptosis, membrane asymmetry is lost and PS translocates to the external leaflet. Fluorochrome-labeled Annexin V can then be used to specifically target and identify apoptotic cells. Annexin V Binding Buffer is recommended for use with Annexin V staining. Annexin V binding alone cannot differentiate between apoptotic and necrotic cells. To help distinguish between the necrotic and apoptotic cells we recommend use of our Propidium Iodide Solution (PI). Early apoptotic cells will exclude PI, while late stage apoptotic cells and necrotic cells will stain positively, due to the passage of these dyes into the nucleus where they bind to DNA. Propidium iodide is a fluorescent dye that binds to DNA. When excited by 488 nm laser light, it can be detected with in the PE/Texas Red® channel with a bandpass filter 610/10. It is commonly used in evaluation of cell viability or DNA content in cell cycle analysis by flow cytometry.

Bestandteile: APC Annexin V Apoptosis Detection Kit with PI

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Vorsichtsmaßnahmen: Caution: Propidium Iodide Solution is toxigenic and mutagenic, handle with care.

Handhabung: Do not freeze.

Lagerung: 4 °C

Informationen zur Lagerung: Store between 2°C and 8°C.

Antigendetails

Target: Annexin V (ANXA5) (Annexin A5 (ANXA5))

Andere Bezeichnung: Annexin V (ANXA5 Produkte)