RFP Western Blot Kit: for RFP Chemiluminescent Western Blotting

Produktnummer ABIN2669872

Produktdetails

Target: RFP (Red Fluorescent Protein (RFP))

Reaktivität: Discosoma

Klonalität: Polyklonal

Applikation: Dot Blot (DB), Western Blotting (WB)

Kreuzreaktivität: Cherry, Tomate (Solanum lycopersicum), Banane, Orange, Plum, Strawberry

Anwendungsinformationen

Applikationshinweise: Use Anti-RFP Chemiluminescent Kit for Western Blotting for detection of RFP-tagged recombinant proteins by western blot. his kit is useful for both western blotting and dot blotting methods. Please read the entire product insert prior to use.

Kommentare:

Detection Kit Type: Chemiluminescent Western Blot Kit

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: Wash buffers MUST NOT contain SODIUM AZIDE or other inhibitors of peroxidase activity!

Lagerung: RT/4 °C

Antigendetails

Target: RFP (Red Fluorescent Protein (RFP))

Andere Bezeichnung: DsRed Fluorescent Protein (RFP Produkte)

Hintergrund: Red Fluorescent Protein (RFP) Western Bloting kit allows for the detection of RFP-tagged recombinant proteins present in cell lysates provided by the user. After protein separation by SDS-PAGE and transfer, the membrane is probed with optimized Anti-RFP antibody. Detection of the membrane bound antibody-antigen complex is achieved by the addition of a secondary antibody conjugated to the enzyme horseradish peroxidase. The enzyme reacts with a specialized formulation of luminol, an extremely sensitive, non-radioactive substrate that emits light and allows visualization using X-ray film or other imaging methods, including highly sensitive CCD cameras and imaging systems.
Synonyms: Western Blotting Kit, Chemiluminescent Kit, Peroxidase Kit, Immunoblotting Kit, RFP, rRFP, mCherry, tdTomato, mBanana, mOrange, mPlum, mOrange and mStrawberry