Alkaline Phosphatase ELISA Kit

Produktnummer ABIN2702814

Produktdetails Alkaline Phosphatase ELISA Kit

Target: Alkaline Phosphatase (ALP)

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 2-500 MioU/mL

Untere Nachweisgrenze: 2 MioU/mL

Applikation: ELISA

Verwendungszweck: Human Alkaline Phosphatase (ALPL) ELISA Kit for cell culture supernatants, plasma, and serum samples.

Proben: Cell Culture Supernatant, Plasma, Serum

Analytische Methode: Quantitative

Spezifität: This ELISA antibody pair detects human ALKP. Other species not determined.

Sensitivität: 2 mIU/mL

Produktmerkmale:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Bestandteile:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Benötigtes Material:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Anwendungsinformationen

Applikationshinweise: Recommended Dilution for serum and plasma samples2 fold

Probenmenge: 100 μL

Plattentyp: Pre-coated

Protokoll:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: -20 °C

Informationen zur Lagerung: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Haltbarkeit: 6 months

Details zu Alkaline Phosphatase

Target: Alkaline Phosphatase (ALP)

Andere Bezeichnung: Alkaline Phosphatase (ALP Produkte)

Synonyme: ALP ELISA Kit, ALP1 ELISA Kit, CRASH ELISA Kit, ECK0378 ELISA Kit, JW0374 ELISA Kit, psiA ELISA Kit, 2410004D18Rik ELISA Kit, AU040643 ELISA Kit, AW060375 ELISA Kit, ILC ELISA Kit, CTACK ELISA Kit, ESkine ELISA Kit, Akp2 ELISA Kit, alp ELISA Kit, zgc:56672 ELISA Kit, DDBDRAFT_0205491 ELISA Kit, DDBDRAFT_0231570 ELISA Kit, DDB_0205491 ELISA Kit, DDB_0231570 ELISA Kit, Actn2lp ELISA Kit, Alp ELISA Kit, asparaginase like 1 ELISA Kit, bacterial alkaline phosphatase ELISA Kit, C-C motif chemokine ligand 27 ELISA Kit, alkaline phosphatase, liver/bone/kidney ELISA Kit, alkaline phosphatase ELISA Kit, PDZ and LIM domain 3 ELISA Kit, ASRGL1 ELISA Kit, phoA ELISA Kit, Asrgl1 ELISA Kit, CCL27 ELISA Kit, alpl ELISA Kit, alp ELISA Kit, Pdlim3 ELISA Kit

Hintergrund: Gene Names: ALPL
Protein names: Alkaline phosphatase, tissue-nonspecific isozyme (AP-TNAP) (TNSALP) (EC 3.1.3.1) (Alkaline phosphatase liver/bone/kidney isozyme)

Gen-ID: 249

UniProt: P05186