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LY9 ELISA Kit
CD229
Reaktivität: Human
Colorimetric
Sandwich ELISA
80-20.000 pg/mL
Cell Culture Supernatant, Plasma, Serum
Produktnummer ABIN2702892
Produktdetails LY9 ELISA Kit
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Target
Alle LY9 (CD229) ELISA Kits anzeigen
LY9 (CD229)
(Lymphocyte Antigen 9 (CD229))
Reaktivität
Alle Reaktivitäten für LY9 ELISA Kits
Human
Nachweismethode
Colorimetric
Methodentyp
Sandwich ELISA
Detektionsbereich
80-20.000 pg/mL
Untere Nachweisgrenze
80 pg/mL
Applikation
ELISA
Verwendungszweck
Human CD229 (SLAMF3) ELISA Kit for cell culture supernatants, plasma, and serum samples.
Proben
Cell Culture Supernatant, Plasma, Serum
Analytische Methode
Quantitative
Spezifität
Cross Reactivity: This ELISA kit shows no cross-reactivity with the following cytokines tested: human ADAM12, ADAM8, B7-H3, BMPR-IB, Cadherin-13, Cadherin-4, CD155, CD48, CD58, CD84, CD99, CEACAM-5, CF XIV, Cystatin A, Cystatin B, Cystatin E/M, Desmoglein 2, DR3, ErbB4, ESAM, FGF-21, Galectin-2, Galectin-9, ICOS, JAM-A, JAM-B, Kallikrein 5, Midkine, Pentraxin 3, Pref-1, Siglec-10, SLAM, SP-D, Syndecan-4, Testican 2, TIM-3, TLR4, TRAIL, ULBP-1 .
Sensitivität
80 pg/mL
Produktmerkmale
Strip plates and additional reagents allow for use in multiple experiments Quantitative protein detection Establishes normal range The best products for confirmation of antibody array data
Bestandteile
Pre-Coated 96-well Strip Microplate Wash Buffer Stop Solution Assay Diluent(s) Lyophilized Standard Biotinylated Detection Antibody Streptavidin-Conjugated HRP TMB One-Step Substrate
Benötigtes Material
Distilled or deionized water Precision pipettes to deliver 2 μL to 1 μL volumes Adjustable 1-25 μL pipettes for reagent preparation 100 μL and 1 liter graduated cylinders Tubes to prepare standard and sample dilutions Absorbent paper Microplate reader capable of measuring absorbance at 450nm Log-log graph paper or computer and software for ELISA data analysis
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Anwendungsinformationen
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Applikationshinweise
Recommended Dilution for serum and plasma samples3 fold
Probenmenge
100 μL
Plattentyp
Pre-coated
Protokoll
Prepare all reagents, samples and standards as instructed in the manual. Add 100 μL of standard or sample to each well. Incubate 2.5 h at RT or O/N at 4 °C. Add 100 μL of prepared biotin antibody to each well. Incubate 1 h at RT. Add 100 μL of prepared Streptavidin solution to each well. Incubate 45 min at RT. Add 100 μL of TMB One-Step Substrate Reagent to each well. Incubate 30 min at RT. Add 50 μL of Stop Solution to each well. Read at 450 nm immediately.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Handhabung
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Lagerung
-20 °C
Informationen zur Lagerung
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Haltbarkeit
6 months
Details zu LY9
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Target
Alle LY9 (CD229) ELISA Kits anzeigen
LY9 (CD229)
(Lymphocyte Antigen 9 (CD229))
Andere Bezeichnung
CD229 (CD229 Produkte )
Synonyme
LY9 ELISA Kit, CD229 ELISA Kit, SLAMF3 ELISA Kit, hly9 ELISA Kit, mLY9 ELISA Kit, AI893573 ELISA Kit, Lgp100 ELISA Kit, Ly-9 ELISA Kit, T100 ELISA Kit, lymphocyte antigen 9 ELISA Kit, LY9 ELISA Kit, Ly9 ELISA Kit
Hintergrund
Gene Names: LY9 CDABP0070 Protein names: T-lymphocyte surface antigen Ly-9 (Cell surface molecule Ly-9) (Lymphocyte antigen 9) (SLAM family member 3) (SLAMF3) (Signaling lymphocytic activation molecule 3) (CD antigen CD229)
Gen-ID
4063
UniProt
Q9HBG7
Pathways
BCR Signaling
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