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LY9 ELISA Kit

CD229 Reaktivität: Human Colorimetric Sandwich ELISA 80-20.000 pg/mL Cell Culture Supernatant, Plasma, Serum
Produktnummer ABIN2702892
  • Target Alle LY9 (CD229) ELISA Kits anzeigen
    LY9 (CD229) (Lymphocyte Antigen 9 (CD229))
    Reaktivität
    • 3
    • 1
    Human
    Nachweismethode
    Colorimetric
    Methodentyp
    Sandwich ELISA
    Detektionsbereich
    80-20.000 pg/mL
    Untere Nachweisgrenze
    80 pg/mL
    Applikation
    ELISA
    Verwendungszweck
    Human CD229 (SLAMF3) ELISA Kit for cell culture supernatants, plasma, and serum samples.
    Proben
    Cell Culture Supernatant, Plasma, Serum
    Analytische Methode
    Quantitative
    Spezifität
    Cross Reactivity: This ELISA kit shows no cross-reactivity with the following cytokines tested: human ADAM12, ADAM8, B7-H3, BMPR-IB, Cadherin-13, Cadherin-4, CD155, CD48, CD58, CD84, CD99, CEACAM-5, CF XIV, Cystatin A, Cystatin B, Cystatin E/M, Desmoglein 2, DR3, ErbB4, ESAM, FGF-21, Galectin-2, Galectin-9, ICOS, JAM-A, JAM-B, Kallikrein 5, Midkine, Pentraxin 3, Pref-1, Siglec-10, SLAM, SP-D, Syndecan-4, Testican 2, TIM-3, TLR4, TRAIL, ULBP-1 .
    Sensitivität
    80 pg/mL
    Produktmerkmale
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data
    Bestandteile
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    Benötigtes Material
    • Distilled or deionized water
    • Precision pipettes to deliver 2 μL to 1 μL volumes
    • Adjustable 1-25 μL pipettes for reagent preparation
    • 100 μL and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
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  • Applikationshinweise
    Recommended Dilution for serum and plasma samples3 fold
    Probenmenge
    100 μL
    Plattentyp
    Pre-coated
    Protokoll
    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 μL of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4 °C.
    4. Add 100 μL of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 μL of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Lagerung
    -20 °C
    Informationen zur Lagerung
    The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
    Haltbarkeit
    6 months
  • Target Alle LY9 (CD229) ELISA Kits anzeigen
    LY9 (CD229) (Lymphocyte Antigen 9 (CD229))
    Andere Bezeichnung
    CD229 (CD229 Produkte)
    Synonyme
    LY9 ELISA Kit, CD229 ELISA Kit, SLAMF3 ELISA Kit, hly9 ELISA Kit, mLY9 ELISA Kit, AI893573 ELISA Kit, Lgp100 ELISA Kit, Ly-9 ELISA Kit, T100 ELISA Kit, lymphocyte antigen 9 ELISA Kit, LY9 ELISA Kit, Ly9 ELISA Kit
    Hintergrund
    Gene Names: LY9 CDABP0070
    Protein names: T-lymphocyte surface antigen Ly-9 (Cell surface molecule Ly-9) (Lymphocyte antigen 9) (SLAM family member 3) (SLAMF3) (Signaling lymphocytic activation molecule 3) (CD antigen CD229)
    Gen-ID
    4063
    UniProt
    Q9HBG7
    Pathways
    BCR Signaling
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