CHI3L1 ELISA Kit

Produktnummer ABIN2702910

Produktdetails CHI3L1 ELISA Kit

Target: CHI3L1 (Chitinase 3-Like 1 (Cartilage Glycoprotein-39) (CHI3L1))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 6-1.500 pg/mL

Untere Nachweisgrenze: 6 pg/mL

Applikation: ELISA

Verwendungszweck: Human CHI3L1 ELISA Kit for cell culture supernatants, plasma, and serum samples.

Proben: Cell Culture Supernatant, Plasma, Serum

Analytische Methode: Quantitative

Spezifität: This ELISA antibody pair detects human CHI3L1. Other species not determined.

Sensitivität: 6 pg/mL

Produktmerkmale:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Bestandteile:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Benötigtes Material:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Anwendungsinformationen

Applikationshinweise: Recommended Dilution for serum and plasma samples150 fold

Probenmenge: 100 μL

Plattentyp: Pre-coated

Protokoll:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: -20 °C

Informationen zur Lagerung: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Haltbarkeit: 6 months

Details zu CHI3L1

Target: CHI3L1 (Chitinase 3-Like 1 (Cartilage Glycoprotein-39) (CHI3L1))

Andere Bezeichnung: CHI3L1 (CHI3L1 Produkte)

Synonyme: AW208766 ELISA Kit, Brp39 ELISA Kit, Gp39 ELISA Kit, ASRT7 ELISA Kit, CGP-39 ELISA Kit, GP-39 ELISA Kit, GP39 ELISA Kit, HC-gp39 ELISA Kit, HCGP-3P ELISA Kit, YKL-40 ELISA Kit, YKL40 ELISA Kit, YYL-40 ELISA Kit, hCGP-39 ELISA Kit, GP38K ELISA Kit, CLP-1 ELISA Kit, SPC-40 ELISA Kit, SPS-40 ELISA Kit, Chil1 ELISA Kit, BP40 ELISA Kit, MGP-40 ELISA Kit, chitinase-like 1 ELISA Kit, chitinase 3 like 1 ELISA Kit, chitinase 3-like 1 (cartilage glycoprotein-39) ELISA Kit, Chil1 ELISA Kit, CHI3L1 ELISA Kit, Chi3l1 ELISA Kit

Substanzklasse: Viral Protein

Hintergrund: Gene Names: CHI3L1
Protein names: Chitinase-3-like protein 1 (39 kDa synovial protein) (Cartilage glycoprotein 39) (CGP-39) (GP-39) (hCGP-39) (YKL-40)

Gen-ID: 1116

UniProt: P36222