FAP ELISA Kit

Produktnummer ABIN2702993

Produktdetails FAP ELISA Kit

Target: FAP (Fibroblast Activation Protein, alpha (FAP))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 12-4000 pg/mL

Untere Nachweisgrenze: 12 pg/mL

Applikation: ELISA

Verwendungszweck: Human FAP ELISA Kit for cell culture supernatants, plasma, and serum samples.

Proben: Plasma, Cell Culture Supernatant, Serum

Analytische Methode: Quantitative

Spezifität: This ELISA antibody pair detects human FAP. Other species not determined yet.

Sensitivität: 12 pg/mL

Produktmerkmale:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Bestandteile:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Benötigtes Material:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Anwendungsinformationen

Applikationshinweise: Recommended Dilution for serum and plasma samples200 fold

Probenmenge: 100 μL

Plattentyp: Pre-coated

Protokoll:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: -20 °C

Informationen zur Lagerung: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Haltbarkeit: 6 months

Details zu FAP

Target: FAP (Fibroblast Activation Protein, alpha (FAP))

Andere Bezeichnung: FAP (FAP Produkte)

Synonyme: fap-A ELISA Kit, gene 13 ELISA Kit, FAP ELISA Kit, fapa ELISA Kit, dppiv ELISA Kit, seprase ELISA Kit, DPPIV ELISA Kit, FAPA ELISA Kit, fibroblast activation protein ELISA Kit, fibroblast activation protein alpha L homeolog ELISA Kit, fibroblast activation protein alpha ELISA Kit, fibroblast activation protein, alpha ELISA Kit, prolyl endopeptidase FAP ELISA Kit, Fap ELISA Kit, fap.L ELISA Kit, FAP ELISA Kit, fap ELISA Kit, LOC100547273 ELISA Kit

Hintergrund: Gene Names: FAP
Protein names: Prolyl endopeptidase FAP (EC 3.4.21.26) (170 kDa melanoma membrane-bound gelatinase) (Dipeptidyl peptidase FAP) (EC 3.4.14.5) (Fibroblast activation protein alpha) (FAPalpha) (Gelatine degradation protease FAP) (EC 3.4.21.-) (Integral membrane serine protease) (Post-proline cleaving enzyme) (Serine integral membrane protease) (SIMP) (Surface-expressed protease) (Seprase) [Cleaved into: Antiplasmin-cleaving enzyme FAP, soluble form (APCE) (EC 3.4.14.5) (EC 3.4.21.-) (EC 3.4.21.26)]

Gen-ID: 2191

UniProt: Q12884

Pathways: Tube Formation