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IFNGR1 ELISA Kit
IFNGR1
Reaktivität: Maus
Colorimetric
Sandwich ELISA
0.16-40 ng/mL
Cell Culture Supernatant, Plasma, Serum
Produktnummer ABIN4885547
Produktdetails IFNGR1 ELISA Kit
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Target
Alle IFNGR1 ELISA Kits anzeigen
IFNGR1
(Interferon gamma Receptor 1 (IFNGR1))
Reaktivität
Alle Reaktivitäten für IFNGR1 ELISA Kits
Maus
Nachweismethode
Colorimetric
Methodentyp
Sandwich ELISA
Detektionsbereich
0.16-40 ng/mL
Untere Nachweisgrenze
0.16 ng/mL
Applikation
ELISA
Verwendungszweck
Mouse IFN-gamma R1 ELISA Kit for Cell Culture Supernatants, Plasma, and Serum samples.
Proben
Cell Culture Supernatant, Plasma, Serum
Analytische Methode
Quantitative
Spezifität
The antibody pair provided in this kit recognizes mouse IFN-gamma R1.
Sensitivität
0.16 ng/mL
Produktmerkmale
Strip plates and additional reagents allow for use in multiple experiments Quantitative protein detection Establishes normal range The best products for confirmation of antibody array data
Bestandteile
Pre-Coated 96-well Strip Microplate Wash Buffer Stop Solution Assay Diluent(s) Lyophilized Standard Biotinylated Detection Antibody Streptavidin-Conjugated HRP TMB One-Step Substrate
Benötigtes Material
Distilled or deionized water Precision pipettes to deliver 2 μL to 1 μL volumes Adjustable 1-25 μL pipettes for reagent preparation 100 μL and 1 liter graduated cylinders Tubes to prepare standard and sample dilutions Absorbent paper Microplate reader capable of measuring absorbance at 450nm Log-log graph paper or computer and software for ELISA data analysis
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Anwendungsinformationen
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Applikationshinweise
Recommended Dilution for serum and plasma samples2 fold
Probenmenge
100 μL
Plattentyp
Pre-coated
Protokoll
Prepare all reagents, samples and standards as instructed in the manual. Add 100 μL of standard or sample to each well. Incubate 2.5 h at RT or O/N at 4 °C. Add 100 μL of prepared biotin antibody to each well. Incubate 1 h at RT. Add 100 μL of prepared Streptavidin solution to each well. Incubate 45 min at RT. Add 100 μL of TMB One-Step Substrate Reagent to each well. Incubate 30 min at RT. Add 50 μL of Stop Solution to each well. Read at 450 nm immediately.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Handhabung
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Lagerung
-20 °C
Informationen zur Lagerung
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Haltbarkeit
6 months
Details zu IFNGR1
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Target
Alle IFNGR1 ELISA Kits anzeigen
IFNGR1
(Interferon gamma Receptor 1 (IFNGR1))
Andere Bezeichnung
IFN-gamma R1 (IFNGR1 Produkte )
Synonyme
CD119 ELISA Kit, IFNGR ELISA Kit, IFN-gammaR ELISA Kit, Ifgr ELISA Kit, Ifngr ELISA Kit, Nktar ELISA Kit, IFNGR1 ELISA Kit, MGC129098 ELISA Kit, ifngr1 ELISA Kit, MGC194858 ELISA Kit, MGC194873 ELISA Kit, zgc:194858 ELISA Kit, cd119 ELISA Kit, ifngr ELISA Kit, DKFZp469K1232 ELISA Kit, interferon gamma receptor 1 ELISA Kit, cytokine receptor family member B17 ELISA Kit, IFNGR1 ELISA Kit, Ifngr1 ELISA Kit, crfb17 ELISA Kit, ifngr1 ELISA Kit
Hintergrund
Gene Names: Ifngr1 Ifngr Protein names: Interferon gamma receptor 1 (IFN-gamma receptor 1) (IFN-gamma-R1) (CD antigen CD119)
Gen-ID
15979
UniProt
P15261
Pathways
Interferon-gamma Pathway
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