IL-25 ELISA Kit
-
- Target Alle IL-25 (IL25) ELISA Kits anzeigen
- IL-25 (IL25) (Interleukin 25 (IL25))
-
Reaktivität
- Human
- Nachweismethode
- Colorimetric
- Methodentyp
- Sandwich ELISA
- Detektionsbereich
- 0.4-100 ng/mL
- Untere Nachweisgrenze
- 0.4 ng/mL
- Applikation
- ELISA
- Verwendungszweck
- Human IL-17E (IL-25) ELISA Kit for cell culture supernatants, plasma, and serum samples.
- Proben
- Cell Culture Supernatant, Plasma, Serum
- Analytische Methode
- Quantitative
- Spezifität
- Cross Reactivity: This ELISA kit shows no cross-reactivity with the following cytokines tested: human ADAMTS13, Aggrecan, Angiotensinogen, B7-H1, BMPR-IA, BMPR-II, Cadherin-11, CD27, CD6, Ck beta 8-1 , CNTF, DNAM-1, EMMPRIN, FLRG, Follistatin-like 1, Fractalkine, Galectin-1, GITR Ligand, Granulysin, IL-1 R3, IL-15 R, IL-32 alpha, L1CAM-2, LRIG3, LRP-6, MEPE, Nectin-4, Periostin, Persephin, Renin, RGM-B, ROBO3, S100A8, Siglec-7, Syndecan-3, Thrombospondin-2, Thrombospondin-5, Tie-1, ULBP-2.
- Sensitivität
- 0.4 ng/mL
- Produktmerkmale
-
- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
- Bestandteile
-
- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
- Benötigtes Material
-
- Distilled or deionized water
- Precision pipettes to deliver 2 μL to 1 μL volumes
- Adjustable 1-25 μL pipettes for reagent preparation
- 100 μL and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
- Featured
- Zu unserem meistverkauften IL25 ELISA Kit
- Top Product
- Discover our top product IL25 ELISA Kit
-
-
- Applikationshinweise
- Recommended Dilution for serum and plasma samples2 fold
- Probenmenge
- 100 μL
- Plattentyp
- Pre-coated
- Protokoll
-
- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 μL of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4 °C.
- Add 100 μL of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 μL of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 μL of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 μL of Stop Solution to each well.
- Read at 450 nm immediately.
- Beschränkungen
- Nur für Forschungszwecke einsetzbar
-
- Lagerung
- -20 °C
- Informationen zur Lagerung
- The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
- Haltbarkeit
- 6 months
-
-
Interleukin-25 promotes basic fibroblast growth factor expression by human endothelial cells through interaction with IL-17RB, but not IL-17RA." in: Clinical and experimental allergy : journal of the British Society for Allergy and Clinical Immunology, Vol. 42, Issue 11, pp. 1604-14, (2013) (PubMed).
: "
-
Interleukin-25 promotes basic fibroblast growth factor expression by human endothelial cells through interaction with IL-17RB, but not IL-17RA." in: Clinical and experimental allergy : journal of the British Society for Allergy and Clinical Immunology, Vol. 42, Issue 11, pp. 1604-14, (2013) (PubMed).
-
- Target Alle IL-25 (IL25) ELISA Kits anzeigen
- IL-25 (IL25) (Interleukin 25 (IL25))
- Andere Bezeichnung
- IL-17 E (IL25 Produkte)
- Synonyme
- IL17E ELISA Kit, IL-17e ELISA Kit, Il17e ELISA Kit, IL25 ELISA Kit, RGD1561632 ELISA Kit, interleukin 25 ELISA Kit, IL25 ELISA Kit, Il25 ELISA Kit
- Hintergrund
-
Gene Names: IL25 IL17E UNQ3120/PRO10272
Protein names: Interleukin-25 (IL-25) (Interleukin-17E) (IL-17E)
- Gen-ID
- 64806
- UniProt
- Q9H293
-