IL-2 ELISA Kit
-
- Target Alle IL-2 (IL2) ELISA Kits anzeigen
- IL-2 (IL2) (Interleukin 2 (IL2))
-
Reaktivität
- Cat
- Nachweismethode
- Colorimetric
- Methodentyp
- Sandwich ELISA
- Detektionsbereich
- 25-6000 pg/mL
- Untere Nachweisgrenze
- 25 pg/mL
- Applikation
- ELISA
- Verwendungszweck
- Feline IL-2 ELISA Kit for cell culture supernatants, plasma, and serum samples.
- Proben
- Cell Culture Supernatant, Plasma, Serum
- Analytische Methode
- Quantitative
- Spezifität
- This ELISA antibody pair detects feline IL-2. Approximately 3 % cross-reactivity with recombinant canine IL-2 is observed, approximately 1 % cross reactivity with recombinant porcine IL-2 and recombinant human IL-2 is observed.
- Sensitivität
- 25 pg/mL
- Produktmerkmale
-
- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
- Bestandteile
-
- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
- Benötigtes Material
-
- Distilled or deionized water
- Precision pipettes to deliver 2 μL to 1 μL volumes
- Adjustable 1-25 μL pipettes for reagent preparation
- 100 μL and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
- Featured
- Zu unserem meistverkauften IL2 ELISA Kit
- Top Product
- Discover our top product IL2 ELISA Kit
-
-
- Applikationshinweise
- Recommended Dilution for serum and plasma samples2 fold
- Probenmenge
- 100 μL
- Plattentyp
- Pre-coated
- Protokoll
-
- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 μL of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4 °C.
- Add 100 μL of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 μL of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 μL of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 μL of Stop Solution to each well.
- Read at 450 nm immediately.
- Beschränkungen
- Nur für Forschungszwecke einsetzbar
-
- Lagerung
- -20 °C
- Informationen zur Lagerung
- The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
- Haltbarkeit
- 6 months
-
- Target Alle IL-2 (IL2) ELISA Kits anzeigen
- IL-2 (IL2) (Interleukin 2 (IL2))
- Andere Bezeichnung
- IL-2 (IL2 Produkte)
- Synonyme
- IL2 ELISA Kit, IL-2 ELISA Kit, il2 ELISA Kit, TCGF ELISA Kit, lymphokine ELISA Kit, Il-2 ELISA Kit, POIL2 ELISA Kit, interleukin 2 ELISA Kit, interleukin-15 ELISA Kit, IL2 ELISA Kit, il2 ELISA Kit, LOC100303688 ELISA Kit, Il2 ELISA Kit
- Hintergrund
-
Gene Names: IL2
Protein names: Interleukin-2 (IL-2) (T-cell growth factor) (TCGF)
- Gen-ID
- 751114
- UniProt
- Q07885
- Pathways
- JAK-STAT Signalweg, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Activated T Cell Proliferation
-